Characterization of the resistance class 1 integrons in Staphylococcus aureus isolates from milk of lactating dairy cattle in Northwestern China

BMC Veterinary Research
Longping Li, Xin Zhao

Abstract

Integrons are mobile DNA elements and they have an important role in acquisition and dissemination of antimicrobial resistance genes. However, there are limited data available on integrons of Staphylococcus aureus (S. aureus) from bovine mastitis, especially from Chinese dairy cows. To address this knowledge gap, bovine mastitis-inducing S. aureus isolates were investigated for the presence of integrons as well as characterization of gene cassettes. Integrons were detected using PCR reactions and then further characterized by a restriction fragment-length polymorphism analysis and amplicon sequencing. All 121 S. aureus isolates carried the class 1 integrase gene intI1, with no intI2 and intI3 genes detected. One hundred and three isolates were positive for the presence of 12 resistance genes, either alone or in combination with other gene cassettes. These resistance genes encoded resistance to trimethoprim (dhfrV, dfrA1, dfrA12), aminoglycosides (aadA1, aadA5, aadA4, aadA24, aacA4, aadA2, aadB), chloramphenicol (cmlA6) and quaternary ammonium compound (qacH) and were organized into 11 different gene cassettes arrangements (A-K). The gene cassette arrays dfrA1-aadA1 (D, 44.6%), aadA2 (K, 31.4%), dfrA12-orfX2-aadA2 (G, 27.3%) and...Continue Reading

References

Jan 1, 1995·Antimicrobial Agents and Chemotherapy·C LévesqueP H Roy
Oct 6, 1999·Current Opinion in Microbiology·D A Rowe-Magnus, D Mazel
Aug 28, 2002·International Journal of Medical Microbiology : IJMM·Dean A Rowe-Magnus, Didier Mazel
Apr 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sobhan NandiAnne O Summers
Jul 18, 2006·Nature Reviews. Microbiology·Didier Mazel
Sep 7, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Z XuS Yamasaki
May 7, 2009·FEMS Microbiology Reviews·Sally R PartridgeJonathan R Iredell
Jul 30, 2010·Cellular and Molecular Life Sciences : CMLS·Natalia Malachowa, Frank R DeLeo
Apr 28, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Z XuL Shi
Jul 24, 2012·International Journal of Antimicrobial Agents·Kuihai WuYongyu Rui
May 23, 2014·Microbiology and Molecular Biology Reviews : MMBR·Michael R Gillings
May 29, 2014·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Farzaneh FiroozehFereshteh Shahcheraghi
Apr 18, 2017·Current Opinion in Microbiology·Michael R Gillings

❮ Previous
Next ❯

Citations

Jun 18, 2020·International Journal of Molecular Sciences·Ewelina KwiecieńMagdalena Rzewuska
Dec 26, 2018·Journal of Dairy Science·Julie Côté-Gravel, François Malouin
Nov 25, 2020·Journal of Proteome Research·Chao WangQing-Lei Meng

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.