Characterization of the rpsL and rrs genes of streptomycin-resistant clinical isolates of Mycobacterium tuberculosis in Japan

Journal of Applied Microbiology
Chihiro KatsukawaY Suzuki

Abstract

Mutations in the rpsL and rrs genes associated with streptomycin resistance in Mycobacterium tuberculosis clinically isolated in Japan were characterized. The rpsL genes of 172 clinical isolates were amplified by PCR and classified into two groups on the basis of MboII restriction digestion. Thirty-three out of 54 (61.1%) streptomycin-highly resistant isolates (MIC > 200 micrograms ml-1) were not digested by MboII. By contrast, the remaining 21 of 54 (38.9%) streptomycin-highly resistant isolates, all of 41 isolates with streptomycin resistance at a lower level (20 micrograms ml-1 < MIC < or = 200 micrograms ml-1), and all of 77 streptomycin-sensitive isolates, were restricted. Thus, all isolates resistant for MboII digestion showed a high level of resistance to streptomycin. Subsequently, the sequence for the rpsL and rrs genes from the 46 isolates were analysed. Eighteen out of 19 (94.7%) streptomycin-highly resistant isolates carried a mutation in any rpsL gene at position 43 or 88, or the rrs gene; 10 out of 17 (58.8%) streptomycin-resistant isolates at a lower level were confirmed to exhibit the mutation of either the mutated rpsL gene at position 88, or the rrs gene. In the total 36 streptomycin-resistant isolates, the mu...Continue Reading

Citations

Jan 19, 2013·World Journal of Microbiology & Biotechnology·Hum Nath JnawaliYoung Kil Park
Aug 31, 2010·The Journal of Antimicrobial Chemotherapy·Griselda TudóJulià González-Martín
Feb 5, 2002·Journal of Clinical Microbiology·Yasuhiko SuzukiHajime Oda
Jun 2, 2015·Nihon saikingaku zasshi. Japanese journal of bacteriology·Hiroto ShinomiyaKomei Shirabe
Jun 23, 2007·Tuberculosis·Sang-Nae Cho, Patrick J Brennan
Feb 17, 2006·Annals of the New York Academy of Sciences·J M Rolain, D Raoult
Jun 25, 2013·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yuhki NagaiTetsuo Yamaguchi
Jun 13, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Y-J SunA S G Lee
Apr 14, 2017·Journal of Global Antimicrobial Resistance·Azar Dokht KhosraviHamed Goodarzi
Jun 28, 2007·Yonsei Medical Journal·Sang-Nae Cho
Apr 24, 2007·The Journal of Antimicrobial Chemotherapy·Silpak BiswasJean-Marc Rolain
Feb 16, 2017·Tuberculosis Research and Treatment·Solima M A SabeelBahaeldin K Elamin
May 18, 2019·Journal of Global Antimicrobial Resistance·Feriel BouzianeUNKNOWN CNR-MyRMA
Jun 25, 2015·Microbiology Spectrum·Keira A CohenAlexander S Pym

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.