Characterization of the S-locus region of almond (Prunus dulcis): analysis of a somaclonal mutant and a cosmid contig for an S haplotype.

Genetics
Koichiro UshijimaH Hirano

Abstract

Almond has a self-incompatibility system that is controlled by an S locus consisting of the S-RNase gene and an unidentified "pollen S gene." An almond cultivar "Jeffries," a somaclonal mutant of "Nonpareil" (S(c)S(d)), has a dysfunctional S(c) haplotype both in pistil and pollen. Immunoblot and genomic Southern blot analyses detected no S(c) haplotype-specific signal in Jeffries. Southern blot showed that Jeffries has an extra copy of the S(d) haplotype. These results indicate that at least two mutations had occurred to generate Jeffries: (1) deletion of the S(c) haplotype and (2) duplication of the S(d) haplotype. To analyze the extent of the deletion in Jeffries and gain insight into the physical limit of the S locus region, approximately 200 kbp of a cosmid contig for the S(c) haplotype was constructed. Genomic Southern blot analyses showed that the deletion in Jeffries extends beyond the region covered by the contig. Most cosmid end probes, except those near the S(c)-RNase gene, cross-hybridized with DNA fragments from different S haplotypes. This suggests that regions away from the S(c)-RNase gene can recombine between different S haplotypes, implying that the cosmid contig extends to the borders of the S locus.

References

Oct 1, 1995·Current Opinion in Genetics & Development·J F GolzE Newbigin
Nov 3, 1998·Genetics·W HuJ Messing
Jul 17, 1999·Trends in Plant Science·V Gorbunova, A A Levy
Nov 27, 1999·Science·C R SchopferJ B Nasrallah
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A G McCubbin, T Kao
Oct 25, 2000·Trends in Plant Science·N BrugièreY Cui
Aug 1, 1999·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T EntaniA Isogai

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Citations

Aug 16, 2008·The Journal of Heredity·Jorge VieiraCristina P Vieira
Jul 12, 2008·Journal of Experimental Botany·Tatsuya TsukamotoAmy F Iezzoni
Jul 27, 2004·The Plant Journal : for Cell and Molecular Biology·Koichiro UshijimaRyutaro Tao
Sep 2, 2008·Genetics·Jorge VieiraCristina P Vieira
Jan 5, 2008·Plant Molecular Biology·Kazuma OkadaTakeshi Takasaki-Yasuda
May 25, 2007·The Plant Journal : for Cell and Molecular Biology·Qiuying YangYongbiao Xue
Jan 27, 2005·Biochemical and Biophysical Research Communications·Chengwei ShangHisashi Hirano
Aug 18, 2006·Plant Molecular Biology·Tatsuya TsukamotoAmy F Iezzoni
Sep 21, 2006·The Journal of Heredity·Nathanael R HauckAmy F Iezzoni
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·J F GolzE Newbigin
Mar 8, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tetsuyuki EntaniSeiji Takayama

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