PMID: 3322197Dec 1, 1987Paper

Characterization of the Saccharomyces cerevisiae cis-prenyltransferase required for dolichyl phosphate biosynthesis

Archives of Biochemistry and Biophysics
W L Adair, N Cafmeyer

Abstract

The prenyltransferase involved in the biosynthesis of dolichyl phosphate has been characterized in Saccharomyces cerevisiae. Although the enzyme is predominantly membrane-bound, a significant percentage was found in the soluble fraction. The prenyltransferase preferentially utilizes farnesyl pyrophosphate as the allylic substrate and isopentenyl pyrophosphate as cosubstrate with half-maximal velocities obtained at 25 and 6.7 microM, respectively. The enzymatic activity is sensitive to sulfhydryl reagents and is inhibited by all detergents tested, except 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate at concentrations less than 5 mM. The product of the reaction has been characterized as an alpha-unsaturated polyprenyl pyrophosphate, containing 12-15 isoprene units, approximately two isoprene units shorter than the endogenous yeast dolichyl phosphate. The stereochemistry of addition of isoprene units by the prenyltransferase was shown to be cis by a comparison of the HPLC retention time for a pentadecaprenyl phosphate derived from the in vitro reaction product with that for an authentic mixture of alpha-cis- and alpha-trans-pentadecaprenyl phosphates.

References

Dec 7, 1977·Biochemical and Biophysical Research Communications·D K Grange, W L Adair
Dec 1, 1986·The Biochemical Journal·M Servouse, F Karst
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M E BassonJ Rine
Feb 1, 1986·The Biochemical Journal·K IshiiK Ogura
Feb 1, 1987·Archives of Biochemistry and Biophysics·T BabaC M Allen
Aug 1, 1973·European Journal of Biochemistry·P Jung, W Tanner
Apr 1, 1980·Archives of Biochemistry and Biophysics·T Baba, C M Allen

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Citations

Nov 1, 1989·Chemistry and Physics of Lipids·W L Adair, N Cafmeyer
Jan 1, 1996·Biochimie·A SzkopińskaG Palamarczyk
Jan 8, 1999·Biochimica Et Biophysica Acta·P Burda, M Aebi
Aug 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·L HellerW L Adair
Mar 11, 2006·Fungal Genetics and Biology : FG & B·Urszula Perlińska-LenartJoanna S Kruszewska
Feb 26, 2013·Biochimica Et Biophysica Acta·Martin Loibl, Sabine Strahl
May 8, 2009·Biochimica Et Biophysica Acta·Jonas Denecke, Christian Kranz
Aug 27, 2017·The Journal of Biological Chemistry·Kariona A GrabińskaWilliam C Sessa
Feb 13, 2016·Microbiology and Molecular Biology Reviews : MMBR·Monika SchmollAlfredo Herrera-Estrella
Jul 13, 2016·The Journal of Biological Chemistry·Kariona A GrabińskaWilliam C Sessa
Mar 3, 2012·G3 : Genes - Genomes - Genetics·Laura M Vaughn, Patrick H Masson
Feb 25, 1998·Biochemical and Biophysical Research Communications·S S Krag
Mar 26, 1998·Biochemical and Biophysical Research Communications·G J QuellhorstS S Krag
Oct 1, 1988·Archives of Biochemistry and Biophysics·Z ChenC M Allen

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