PMID: 9428669Jan 15, 1998Paper

Characterization of the Saccharomyces cerevisiae ARG7 gene encoding ornithine acetyltransferase, an enzyme also endowed with acetylglutamate synthase activity

European Journal of Biochemistry
M CrabeelO Soetens

Abstract

We have cloned by functional complementation and characterized the yeast ARG7 gene encoding mitochondrial ornithine acetyltransferase, the enzyme catalyzing the fifth step in arginine biosynthesis. While forming ornithine, this enzyme regenerates acetylglutamate, also produced in the first step by the ARG2-encoded acetylglutamate synthase. Interestingly, total deletion of the genomic ARG7 ORF resulted in an arginine-leaky phenotype, indicating that yeast cells possess an alternative route for generating ornithine from acetylornithine. Yeast ornithine acetyltransferase has been purified and characterized previously as a heterodimer of two subunits proposed to derive from a single precursor protein [Liu, Y-S., Van Heeswijck R., Hoj, P. & Hoogenraad, N. (1995) Eur. J. Biochem. 228, 291-296]; those authors further suggested that the internal processing of Arg7p, which is a mitochondrial enzyme, might occur in the matrix, while the leader peptide would be of the non-cleavable-type. The characterization of the gene (a) establishes that Arg7p is indeed encoded by a single gene, (b) demonstrates the existence of a cleaved mitochondrial prepeptide of eight residues, and (c) shows that the predicted internal processing site is unlike the...Continue Reading

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Citations

Nov 13, 2001·Molecular and Biochemical Parasitology·C GafanB J Berger
May 3, 2002·European Journal of Biochemistry·Nadia J KershawChristopher J Schofield
Dec 5, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Dominique MaesIngrid Zegers
Feb 6, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·R SankaranarayananM N G James
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Ying XuNicolas Glansdorff
Aug 27, 2005·Plant Physiology and Biochemistry : PPB·Robert D Slocum
Aug 19, 2015·Frontiers in Plant Science·Gudrun WinterDietmar Funck
Jun 14, 2012·FEMS Yeast Research·Patrícia de Sousa LimaCélia Maria de Almeida Soares
Sep 16, 2008·Journal of Basic Microbiology·Marcelo BaezaVíctor Cifuentes
Jul 4, 2019·Amino Acids·Daniel Charlier, Indra Bervoets

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