Characterization of the SARS-CoV-2 Neutralization Potential of COVID-19-Convalescent Donors.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Bernd JahrsdörferHubert Schrezenmeier

Abstract

The current SARS-CoV-2 pandemic has triggered the development of various SARS-CoV-2 neutralization tests. A wild-type virus (using African green monkey VeroE6 cells), a pseudovirus (using human Caco-2 cells), and a surrogate neutralization test platform were applied to characterize the SARS-CoV-2 neutralization potential of a cohort of 111 convalescent plasma donors over a period of seven months after diagnosis. This allowed an in-depth validation and assay performance analysis of these platforms. More importantly, we found that SARS-CoV-2 neutralization titers were stable or even increased within the observation period, which contradicts earlier studies reporting a rapid waning of Ab titers after three to four months. Moreover, we observed a positive correlation of neutralization titers with increasing age, number of symptoms reported, and the presence of the Rhesus Ag RhD. Validation of the platforms revealed that highest assay performances were obtained with the wild-type virus and the surrogate neutralization platforms. However, our data also suggested that selection of cutoff titers had a strong impact on the evaluation of neutralization potency. When taking strong neutralization potency, as demonstrated by the wild-type v...Continue Reading

References

Nov 19, 2008·Virology Journal·Petra HerzogMarcel A Müller
Aug 28, 2014·The New England Journal of Medicine·Christian DrostenZiad A Memish
Mar 19, 2020·The New England Journal of Medicine·Xiaoxia LuUNKNOWN Chinese Pediatric Novel Coronavirus Study Team
Jul 14, 2020·Annals of Hematology·Christopher A LatzAnahita Dua
Sep 30, 2020·The New England Journal of Medicine·Evan J AndersonUNKNOWN mRNA-1273 Study Group
Oct 17, 2020·The Journal of Infectious Diseases·Bernd JahrsdörferHubert Schrezenmeier
Oct 22, 2020·Science·Ludovico Cantuti-CastelvetriMikael Simons
Oct 30, 2020·Science·Ania WajnbergCarlos Cordon-Cardo
Nov 4, 2020·Nature·Daniel StadlbauerFlorian Krammer
Nov 25, 2020·The New England Journal of Medicine·Ventura A SimonovichUNKNOWN PlasmAr Study Group
Dec 5, 2020·Journal of Virological Methods·Christine von RheinBarbara S Schnierle
Dec 11, 2020·The New England Journal of Medicine·Fernando P PolackUNKNOWN C4591001 Clinical Trial Group
Mar 21, 2021·Nature Communications·Lukas WettsteinJan Münch
Apr 2, 2021·Cell·Markus HoffmannStefan Pöhlmann

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