Characterization of the sea bass melanocortin 5 receptor: a putative role in hepatic lipid metabolism

The Journal of Experimental Biology
Elisa SánchezJosé Miguel Cerdá-Reverter

Abstract

The melanocortin 5 receptor (MC5R) plays a key role in the regulation of exocrine secretion in mammalian species. This receptor has also been characterized in some fish species but its function is unknown. We report the molecular and pharmacological characterization, as well as the tissue expression pattern, of sea bass MC5R. Cloning of five active alleles showing different levels of sensitivity to endogenous melanocortin and one non-functional allele demonstrate the allelic complexity of the MC5R locus. The sea bass receptor was activated by all the melanocortins tested, with ACTH and desacetyl-MSH and beta-MSH showing the lowest efficiency. The acetylation of the MSH isoforms seems to be critical for the effectiveness of the agonist. Agouti-related protein had no effect on basal or agonist-stimulated activation of the receptor. SbMC5R was mainly expressed in the brain but lower expression levels were found in several peripheral tissues, including liver. Progressive fasting did not induce up- or downregulation of hypothalamic MC5R expression, suggesting that central MC5R is not involved in the regulation of food intake in the sea bass. MTII, a sbMC5R agonist, stimulated hepatic lipolysis in vitro, measured as free fatty acid r...Continue Reading

References

Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Apr 29, 1994·Biochemical and Biophysical Research Communications·N GriffonP Sokoloff
May 16, 1994·Biochemical and Biophysical Research Communications·I GantzT Yamada
Sep 15, 1993·Biochemical and Biophysical Research Communications·V ChhajlaniJ E Wikberg
Jan 1, 1997·Critical Reviews in Neurobiology·M G Castro, E Morrison
Jun 30, 1997·FEBS Letters·H B SchiöthJ E Wikberg
Aug 15, 1998·Molecular and Cellular Endocrinology·H B SchiöthJ E Wikberg
Oct 24, 1998·General and Comparative Endocrinology·S Takeuchi, S Takahashi
Sep 16, 2003·Regulatory Peptides·José Miguel Cerdá-ReverterRichard Ector Peter
Jan 10, 2004·Journal of Neurochemistry·José Miguel Cerdá-ReverterRichard Ector Peter
Nov 18, 2005·General and Comparative Endocrinology·Akiyoshi Takahashi, Hiroshi Kawauchi
Nov 2, 2006·Endocrine Reviews·Roger D Cone
Feb 20, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Elisa SánchezJosé Miguel Cerdá-Reverter

❮ Previous
Next ❯

Citations

Mar 8, 2014·Advances in Chronic Kidney Disease·Rujun Gong
Dec 27, 2011·General and Comparative Endocrinology·Yuki KobayashiAkiyoshi Takahashi
Jan 7, 2011·European Journal of Pharmacology·José Miguel Cerdá-ReverterJosep Rotllant
Feb 25, 2014·General and Comparative Endocrinology·Maria Josep AgulleiroJosé Miguel Cerdá-Reverter
Sep 23, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Duk-Young Kang, Hyo-Chan Kim
Mar 26, 2013·General and Comparative Endocrinology·Akiyoshi TakahashiKanta Mizusawa
Nov 8, 2018·Journal of Molecular Endocrinology·Ming ZhuChao Zhang
Dec 7, 2011·Nature Reviews. Nephrology·Rujun Gong
Feb 6, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Paula Suarez-BreguaJosep Rotllant
Nov 9, 2018·Frontiers in Endocrinology·Marta Conde-SieiraJosé L Soengas
Apr 6, 2020·Cellular and Molecular Life Sciences : CMLS·Yahong XuRujun Gong
Feb 8, 2021·Trends in Ecology & Evolution·M E McNamaraA Roulin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.