Characterization of the second LysR-type regulator in the biphenyl-catabolic gene cluster of Pseudomonas pseudoalcaligenes KF707.

Journal of Bacteriology
Takahito WatanabeK Furukawa

Abstract

Pseudomonas pseudoalcaligenes KF707 possesses a biphenyl-catabolic (bph) gene cluster consisting of bphR1A1A2-(orf3)-bphA3A4BCX0X1X2X3D. The bphR1 (formerly orf0) gene product, which belongs to the GntR family, is a positive regulator for itself and bphX0X1X2X3D. Further analysis in this study revealed that a second regulator belonging to the LysR family (designated bphR2) is involved in the regulation of the bph genes in KF707. The bphR2 gene was not located near the bph gene cluster, and its product (BphR2) exhibited a high level of similarity to NahR (the naphthalene- and salicylate-catabolic regulator belonging to the LysR family) in plasmid NAH7 of Pseudomonas putida. A strain containing a disrupted bphR2 gene failed to grow on biphenyl as a sole source of carbon, and the BphD (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase) activity was significantly reduced compared to that of wild-type strain KF707. Furthermore, the same strain exhibited extremely low transcription of bphR1, bphA1, bphC, bphX0, and bphD. However, when the bphR2 gene was provided in trans to the bphR2-disrupted strain, the transcription level of these genes was restored. These results indicate that bphR2 regulates the bph genes positively as a s...Continue Reading

References

Dec 1, 1988·Journal of Bacteriology·I S YouI C Gunsalus
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M A Schell
Jun 1, 1985·Journal of Bacteriology·K M Yen, I C Gunsalus
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K M Yen, I C Gunsalus
Jan 1, 1993·Annual Review of Microbiology·M A Schell
Jan 11, 2000·Molecular & General Genetics : MGG·S MouzA Toussaint
Jul 20, 2000·The Journal of Biological Chemistry·T WatanabeK Furukawa
Oct 12, 2000·Current Opinion in Biotechnology·E Díaz, M A Prieto
Dec 17, 2002·The Journal of General and Applied Microbiology·Kensuke Furukawa

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Citations

Dec 23, 2004·Applied Microbiology and Biotechnology·Dietmar H Pieper
Aug 5, 2004·Journal of Bacteriology·Kensuke FurukawaMasatoshi Goto
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·David Tropel, Jan Roelof van der Meer
Jun 19, 2008·Journal of Bioscience and Bioengineering·Kensuke Furukawa, Hidehiko Fujihara
Feb 22, 2012·Biochemical and Biophysical Research Communications·Lin BianAi-Sheng Xiong
Nov 22, 2015·Applied and Environmental Microbiology·Matthew J MeierIain B Lambert

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