PMID: 7026242Aug 1, 1981Paper

Characterization of the second prosthetic group in methanol dehydrogenase from hyphomicrobium X

European Journal of Biochemistry
P E VerwielE J Verwiel

Abstract

Procedures are described for preparing 2,7,9-tricarboxy-1H-pyrrolo [2, 3-f]quinoline-4,5-diol (pyrrolo-quinoline quinol) from 2.7,9-tricarboxy-1 H-pyrrolo[2,3-f]quinoline-4,5-dione (pyrrolo-quinoline quinone). When methanol dehydrogenase is denatured, two compounds are liberated which have the same properties as the quinone and quinol mentioned above. On analysing the extract by high-performance liquid chromatography, one molecule of the quinone and one molecule of the quinol per enzyme molecule are found. Mixtures of pyrrolo-quinoline quinone and pyrrolo-quinoline quinol at high pH produce the semiquinone form and, under certain conditions, a diamagnetic complex. Since electron spin resonance (ESR) shows that methanol dehydrogenase contains the semiquinone and the absorption spectrum suggests the presence of a diamagnetic dimer, it is tentatively concluded that the two prosthetic group molecules in the enzyme interact with each other. NMR experiments of pyrrolo-quinoline quinone in 2H2O demonstrate that it is partly hydrated, most probably at the C-5 position. Although methanol adds in the same way, it is still questionable whether the product of this addition plays a role in the mechanism of the enzymic reaction. Potentiometr...Continue Reading

References

Jun 9, 1978·Biochimica Et Biophysica Acta·J A DuineJ Westerling
Apr 13, 1979·Biochemical and Biophysical Research Communications·J WesterlingJ A Duine
Sep 1, 1979·The Biochemical Journal·C W Bamforth, J R Quayle
Aug 30, 1979·Nature·S A SalisburyO Kennard
Jan 1, 1973·Annals of the New York Academy of Sciences·Y P Myer, H A Harbury
Jan 1, 1980·European Journal of Biochemistry·J A DuineP E Verwiel

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Citations

Sep 1, 1989·European Journal of Biochemistry·J FrankJ A Duine
Jan 17, 1983·European Journal of Biochemistry·R de BeerJ Westerling
Jun 3, 2017·Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials·Kazuto IkemotoKazuo Mukai
Jul 6, 2013·Applied Microbiology and Biotechnology·Jessica ZeiserUwe Deppenmeier
Apr 16, 1984·European Journal of Biochemistry·M DijkstraJ A Duine
Feb 20, 2021·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Pedro D Sarmiento-Pavía, Martha E Sosa-Torres
Jan 1, 1987·Free Radical Biology & Medicine·I Yamazaki
May 1, 1989·Applied and Environmental Microbiology·M A van Kleef, J A Duine
Mar 1, 1991·Archives of Biochemistry and Biophysics·F T GreenawayY Zou
May 6, 1986·Biochemistry·M HusainA J Smith
Sep 30, 1986·Biochemical and Biophysical Research Communications·M FaraggiM H Klapper

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