Characterization of the sheep apolipoprotein E (ApoE) gene and allelic variations of the ApoE gene in scrapie Suffolk sheep

Gene
Y KomatsuM Shinagawa

Abstract

Apolipoprotein E (ApoE) plays a central role in lipid transport and is suggested to be involved in neuronal repair. Human ApoE epsilon 4 allele is known as a risk factor for Alzheimer's disease, and an association of the human ApoE genotype with the human prion disease, Creutzfeldt-Jakob disease, is suggested, albeit controversial. We analyzed the sheep ApoE gene to determine whether any association between the sheep ApoE genotype and the sheep prion disease, scrapie, existed. The sheep ApoE cDNA contained an open reading frame (ORF) consisting of 948 base pairs (bp) that encoded 316 amino acids (aa). The sheep ApoE gene was composed of four exons separated by three introns, and the ORF was encoded by three exons, designated exons 2, 3, and 4. Nucleotide sequence analysis also showed the presence of one G/T nucleotide polymorphism in the ORF that resulted in an Ala/Ser amino-acid substitution at codon 258. PCR-restriction fragment length polymorphism analysis of genomic DNA showed the presence of three sheep ApoE genotypes that were the result of the homologous and heterologous combinations of the two alleles. We analyzed the sheep ApoE genotypic and the allelic frequencies in scrapie and control Suffolk sheep, but they did not...Continue Reading

References

May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y K PaikJ M Taylor
Jan 22, 1982·Nucleic Acids Research·S M Mount
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J StrittmatterA D Roses
Oct 20, 1995·Neuroscience Letters·M SalvatoreM Pocchiari
Apr 28, 1997·Biochemical and Biophysical Research Communications·M HoriuchiM Shinagawa

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Citations

Dec 22, 1999·Neurobiology of Aging·C E Finch, R M Sapolsky
Feb 1, 2007·Cell Biochemistry and Function·Murat KasapEmel Ergul

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