PMID: 9525612Apr 3, 1998Paper

Characterization of the single-stranded DNA binding protein encoded by the vaccinia virus I3 gene

Journal of Virology
S C Rochester, Paula Traktman

Abstract

The 34-kDa protein encoded by the I3 gene of vaccinia virus is expressed at early and intermediate times postinfection and is phosphorylated on serine residues. Recombinant I3 has been expressed in Escherichia coli and purified to near homogeneity, as has the protein from infected cells. Both recombinant and endogenous I3 protein demonstrate a striking affinity for single-stranded, but not for double-stranded, DNA. The interaction with DNA is resistant to salt, exhibits low cooperativity, and appears to involve a binding site of approximately 10 nucleotides. Electrophoretic mobility shift assays indicate that numerous I3 molecules can bind to a template, reflecting the stoichiometric interaction of I3 with DNA. Sequence analysis reveals that a pattern of aromatic and charged amino acids common to many replicative single-stranded DNA binding proteins (SSBs) is conserved in I3. The inability to isolate viable virus containing an interrupted I3 allele provides strong evidence that the I3 protein plays an essential role in the viral life cycle. A likely role for I3 as an SSB involved in DNA replication and/or repair is discussed.

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Citations

Sep 6, 2005·Traffic·Birgit Schramm, Jacomine Krijnse Locker
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lichen JingDavid M Koelle
Aug 15, 2008·Journal of Virology·R Jeremy NicholsPaula Traktman
Oct 28, 2011·Journal of Virology·Anna C T C PereiraCláudio A Bonjardim
Apr 10, 2015·Journal of Virology·Kathleen A BoylePaula Traktman
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·S McCraithS Fields
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·P Sanz, B Moss
Jul 29, 2006·Journal of Virology·Zenobia F TaraporewalaJohn T Patton
Jun 18, 2016·Viruses·Jason Liem, Jia Liu
Jul 27, 1999·The Journal of Biological Chemistry·M TsengD H Evans
Apr 23, 2002·Journal of Virology·Massimo MallardoJacomine Krijnse Locker
Oct 26, 2012·Bioinformatics·Darius Kazlauskas, Ceslovas Venclovas
Jul 3, 2015·Journal of Virology·Mona C GjessingOle Bendik Dale
Nov 14, 2017·Nature Communications·Nicolas TarbouriechFrédéric Iseni
Jul 19, 2019·Virus Research·Margarida SimõesFernando Ferreira
Sep 20, 2019·Viruses·Axel KargerMaria Montoya

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