Characterization of the SNAG and SLUG domains of Snail2 in the repression of E-cadherin and EMT induction: modulation by serine 4 phosphorylation.

PloS One
Patricia Molina-OrtizAmparo Cano

Abstract

Snail1 and Snail2, two highly related members of the Snail superfamily, are direct transcriptional repressors of E-cadherin and EMT inducers. Previous comparative gene profiling analyses have revealed important differences in the gene expression pattern regulated by Snail1 and Snail2, indicating functional differences between both factors. The molecular mechanism of Snail1-mediated repression has been elucidated to some extent, but very little is presently known on the repression mediated by Snail2. In the present work, we report on the characterization of Snail2 repression of E-cadherin and its regulation by phosphorylation. Both the N-terminal SNAG and the central SLUG domains of Snail2 are required for efficient repression of the E-cadherin promoter. The co-repressor NCoR interacts with Snail2 through the SNAG domain, while CtBP1 is recruited through the SLUG domain. Interestingly, the SNAG domain is absolutely required for EMT induction while the SLUG domain plays a negative modulation of Snail2 mediated EMT. Additionally, we identify here novel in vivo phosphorylation sites at serine 4 and serine 88 of Snail2 and demonstrate the functional implication of serine 4 in the regulation of Snail2-mediated repressor activity of E...Continue Reading

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Citations

Sep 5, 2012·Cellular and Molecular Life Sciences : CMLS·Ester Sánchez-TillóAntonio Postigo
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Apr 5, 2013·PloS One·Eva CubilloAmparo Cano
Dec 17, 2014·Neoplasia : an International Journal for Oncology Research·Giovanna Ferrari-AmorottiBruno Calabretta
Jun 6, 2019·Scientific Reports·Vignesh SundararajanRuby Yun-Ju Huang
Aug 2, 2020·Science China. Life Sciences·Xiaoyan DaiHongquan Zhang
Sep 13, 2018·PloS One·Cara BickersDavid Traver
Apr 10, 2015·Cancer Research·Reetta VirtakoivuJohanna Ivaska
Jul 22, 2019·Pathology, Research and Practice·Mingzhu ZongLongbang Chen
Dec 4, 2019·Journal of Cell Science·Wenhui ZhouCharlotte Kuperwasser

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Methods Mentioned

BETA
co-immunoprecipitation
electrophoresis
transfection
PCR
transfections
immunoprecipitation

Software Mentioned

Adobe Photoshop

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