Characterization of the soybean genome using EST-derived microsatellite markers

DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes
Hiroshi HisanoSatoshi Tabata

Abstract

We generated a high-density genetic linkage map of soybean using expressed sequence tag (EST)-derived microsatellite markers. A total of 6920 primer pairs (10.9%) were designed to amplify simple sequence repeats (SSRs) from 63,676 publicly available non-redundant soybean ESTs. The polymorphism of two parent plants, the Japanese cultivar 'Misuzudaizu' and the Chinese line 'Moshidou Gong 503', were examined using 10% polyacrylamide gel electrophoresis. Primer pairs showing polymorphism were then used for genotyping 94 recombinant inbred lines (RILs) derived from a cross between the parents. In addition to previously reported markers, 680 EST-derived microsatellite markers were selected and subjected to linkage analysis. As a result, 935 marker loci were mapped successfully onto 20 linkage groups, which totaled 2700.3 cM in length; 693 loci were detected using the 668 EST-derived microsatellite markers developed in this study, the other 242 loci were detected with 105 RFLP markers, 136 genome-derived microsatellite markers, and one phenotypic marker. We examined allelic variation among 23 soybean cultivars/lines and a wild soybean line using 668 mapped EST-derived microsatellite markers (corresponding to 686 marker loci), in order...Continue Reading

References

Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Oct 1, 1994·Genome Génome / Conseil National De Recherches Canada·M MorganteA M Olivieri
Sep 1, 1996·Genetics·R C ShoemakerH R Boerma
May 20, 2000·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·K G HossainK Harada
Sep 20, 2000·The Journal of Heredity·A R FerreiraP Keim
May 12, 2001·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·N YamanakaK Harada
Feb 21, 2002·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·M HayashiK Harada
Apr 19, 2002·Genome Génome / Conseil National De Recherches Canada·Randy ShoemakerCheryl Granger
Aug 15, 2002·Plant Physiology·Anne E HallDaphne Preuss
Aug 28, 2002·Genetics·Andrea PedrosaAndreas Bachmair
Mar 3, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Q J SongP B Cregan
Oct 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hong-Kyu ChoiDouglas R Cook
Jan 5, 2005·Trends in Biotechnology·Rajeev K VarshneyMark E Sorrells
Apr 13, 2005·Plant Physiology·Hongyan ZhuRandy C Shoemaker
Oct 29, 2005·Nature·UNKNOWN International HapMap Consortium
Feb 7, 2006·Current Opinion in Plant Biology·Randy C ShoemakerJeff J Doyle
Jun 14, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Shusei SatoSatoshi Tabata
Feb 9, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jeffry L ShultzDavid A Lightfoot
Jul 20, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E S JonesJ S C Smith
Nov 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P KeimR Freestone

❮ Previous
Next ❯

Citations

Jan 20, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xiaolei WuHenry T Nguyen
Apr 10, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sylvie CloutierRaja Ragupathy
Mar 22, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Paul C KorirJunyi Gai
Jun 18, 2009·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Tae-Young HwangMasao Ishimoto
Apr 2, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takashi SayamaMasao Ishimoto
Jul 20, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Nirala RamchiaryYong Pyo Lim
Dec 6, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Qingzhang DuDeqiang Zhang
Jun 24, 2009·Plant & Cell Physiology·Lakshmi P ManavalanHenry T Nguyen
Jul 10, 2010·Genome Génome / Conseil National De Recherches Canada·S K Gupta, T Gopalakrishna
Aug 19, 2011·Genome Génome / Conseil National De Recherches Canada·B PranaviV Dinesh Kumar
Nov 9, 2012·Breeding Science·Masatsugu HashiguchiRyo Akashi
Nov 9, 2012·Breeding Science·Hideyuki FunatsukiKaien Fujino
May 29, 2009·Genetics·Satoshi WatanabeKyuya Harada
Oct 17, 2014·Breeding Science·Kenta ShirasawaHideki Hirakawa
Dec 4, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yue-Mei ZhaoGui-Fang Zhao
Apr 4, 2013·Journal of Zhejiang University. Science. B·Gu-wen ZhangYa-ming Gong
Jul 21, 2016·Breeding Science·Auchithya DissanayakaRyoji Takahashi
Jan 18, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Loc Van NguyenToshihiro Mochizuki
Apr 10, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sylvie CloutierScott Duguid
Dec 1, 2017·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Satoshi WatanabeAkito Kaga
Feb 18, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yongjun ShuDianjing Guo
Dec 12, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yoshitake TakadaChigen Tsukamoto
Jun 19, 2012·Molecular Breeding : New Strategies in Plant Improvement·Padmalatha KoilkondaSachiko Isobe
Jun 8, 2012·Plant Biotechnology Journal·Shalu JhanwarMukesh Jain
May 1, 2012·Molecular Ecology Resources·Matthew W BlairPrem Sharma
Aug 24, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Changyou LiuShumin Wang

❮ Previous
Next ❯

Datasets Mentioned

BETA
GMES1325
GMES1163
GMES6195
GMES4020
GMES6339
GMES3918
GMES2955
GMES1351

Methods Mentioned

BETA
dissection
PCR
electrophoresis

Software Mentioned

FindPatterns
BLASTN
GCG
Primer3
EXP
MAPMAKER

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes
Tae-Young HwangMasao Ishimoto
DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes
Naoki YamanakaKyuya Harada
DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes
Zhengjun XiaKyuya Harada
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Sylvie CloutierScott Duguid
© 2021 Meta ULC. All rights reserved