PMID: 8608164Mar 29, 1996Paper

Characterization of the sphingomyelin content of isolated pancreatic islets. Evaluation of the role of sphingomyelin hydrolysis in the action of interleukin-1 to induce islet overproduction of nitric oxide

Biochimica Et Biophysica Acta
G KwonJ Turk

Abstract

Inflammatory cytokines may participate in the destruction of pancreatic islets during the pathogenesis of insulin-dependent diabetes mellitus, and the cytokine interleukin-1 (IL-1) strongly inhibits insulin secretion from rat pancreatic islets by a process which involves induction of expression of the inducible isoform of nitric oxide synthase and the overproduction of nitric oxide. The signaling events between IL-1 receptor occupancy and induction of nitric oxide synthase in rat islets involve activation of the transcriptional activator NFkappa B. Because sphingomyelin hydrolysis has been implicated as a signaling process both in NFkappa B activation and in IL-1 action in some cells, we have examined the potential involvement of sphingomyelin hydrolysis in the induction of islet nitric oxide overproduction by IL-1. Rat islet sphingomyelin pools were radiolabeled with [3H]choline, and sphingomyelin was then isolated by normal phase HPLC. Electrospray ionization-mass spectrometric analysis revealed islet sphingomyelin consists of at least 4 distinct molecular species, and the most abundant of them contained sphingosine as the long chain base and a residue of palmitic acid as the fatty acid substituent. Molecular species containi...Continue Reading

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Citations

Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·B BrüggerW D Lehmann
Jun 2, 2007·Progress in Lipid Research·Anthony D PostleGeorge S Attard
Jun 7, 2005·Biochemical Pharmacology·Anjaneyulu Kowluru
May 29, 2003·The Journal of Biological Chemistry·Cynthia L KelpeVincent Poitout
Aug 8, 2020·Cells·Ewa Gurgul-Convey
Jan 13, 1997·Biochemical and Biophysical Research Communications·D BleichJ L Nadler
Apr 29, 2006·Analytical Chemistry·Jeffrey J JonesRichard M O'Brien

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