PMID: 2106508Mar 1, 1990Paper

Characterization of the spoIVB and recN loci of Bacillus subtilis

Journal of Bacteriology
B E Van Hoy, J A Hoch

Abstract

Two independent genes, recN and spoIVB, along with their respective promoter and termination regions, were discovered and sequenced in the 3.4-kilobase region between the ahrC and spoOA genes at map position 216 in the Bacillus subtilis chromosome map. The gene encoding a 576-amino-acid protein, which maintains a high homology with the Escherichia coli recN gene product, was adjacent to ahrC. The sequence revealed a 64,472-dalton polypeptide which contained a conserved ATP-binding site and possible lexA-type regulatory binding sequences in its promoter region. A second open reading frame identified as the spoIVB gene was directly downstream of recN. It consisted of 1,275 nucleotides which coded for a 425-amino-acid polypeptide with a molecular weight of 45,976. Phenotypic, genetic, and transcriptional analyses confirmed that this gene was spoIVB. Although no chloroform-resistant spores were produced by spoIVB-inactivated strains, under microscopic examination, phase-gray forespores were visible. The spoIVB165 mutation was localized to a 200-base-pair region in the amino-terminal portion of the polypeptide, spoIVB was not transcribed until hour 2 of sporulation in wild-type B. subtilis cells, as determined by beta-galactosidase ...Continue Reading

References

Dec 1, 1976·Bacteriological Reviews·P J Piggot, J G Coote
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jan 1, 1976·Advances in Genetics·J A Hoch
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A J Ninfa, B Magasanik
Jul 10, 1987·Nucleic Acids Research·K RostasR G Lloyd
Jan 1, 1986·Annual Review of Genetics·R LosickP J Piggot
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F A FerrariJ A Hoch
Jun 1, 1972·Journal of General Microbiology·J G Coote
Sep 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·P SchaefferJ P Aubert
Jun 1, 1981·Analytical Biochemistry·D S Holmes, M Quigley
Dec 1, 1984·Journal of Bacteriology·P E Love, R E Yasbin
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen

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Citations

Jun 1, 1995·Molecular Microbiology·P BorkP M Gillevet
Apr 1, 1995·Current Microbiology·B W Beall, G N Sanden
Feb 1, 1997·Protein Science : a Publication of the Protein Society·C P Ponting
Mar 1, 1995·Microbiological Reviews·W G Haldenwang
Oct 1, 1992·Current Opinion in Genetics & Development·R G Lloyd, G J Sharples

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