PMID: 9630511Jun 19, 1998Paper

Characterization of the structural and functional changes of hemoglobin in dimethyl sulfoxide by spectroscopic techniques

Biochimica Et Biophysica Acta
C LiuS Dong

Abstract

Circular dichroism (CD), fourier transform infrared (FTIR), and fluorescence spectroscopy were used to explore the effect of dimethyl sulfoxide (DMSO) on the structure and function of hemoglobin (Hb). The native tertiary structure was disrupted completely when the concentration of DMSO reached 50% (v/v), which was determined by loss of the characteristic Soret CD spectrum. Loss of the native tertiary structure could be mainly caused by breaking the hydrogen bonds, between the heme propionate groups and nearby surface amino acid residues, and by disorganizing the hydrophobic interior of this protein. Upon exposure of Hb to 52% DMSO for ca. 12 h in a D2O medium no significant change in 1652 cm-1 band of the FTIR spectrum was produced, which demonstrated that alpha-helical structure predominated. When the concentration of DMSO increased to 57%: (1) the band at 1652 cm-1 disappeared with the appearance of two new bands located at 1661 and 1648 cm-1; (2) another new band at 1623 cm-1 was attributed to the formation of intermolecular beta-sheet or aggregation, which was the direct consequence of breaking of the polypeptide chain by the competition of S&z.dbnd6;O groups in DMSO with C&z.dbnd6;O groups in amide bonds. Further increasin...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D P Flamig, L J Parkhurst
Apr 5, 1979·Journal of Molecular Biology·J Baldwin, C Chothia
Sep 2, 1975·Biochemical and Biophysical Research Communications·F A Ferrone, W C Topp
Jun 26, 1975·Biochimica Et Biophysica Acta·A L Jacobson, P J Krueger
Jun 24, 1991·Biochimica Et Biophysica Acta·M Jackson, H H Mantsch
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S B Ha, G An
Jan 29, 1988·Biochimica Et Biophysica Acta·W K Surewicz, H H Mantsch
Feb 10, 1988·Biochimica Et Biophysica Acta·J L ArrondoH H Mantsch
Jan 1, 1986·Advances in Protein Chemistry·S Krimm, J Bandekar
Jul 18, 1985·Biochimica Et Biophysica Acta·D R Wilson, A H Burr
Oct 1, 1973·Photochemistry and Photobiology·E A BursteinM N Ivkova
Jul 14, 1971·Journal of the American Chemical Society·M C Hsu, R W Woody
Nov 1, 1968·Science·L Stryer
Jul 1, 1984·Journal of Biochemical and Biophysical Methods·J M Purcell, H Susi
Jan 1, 1980·Archives of Biochemistry and Biophysics·C N Pace, H F Marshall
Sep 21, 2004·Chemistry : a European Journal·Maria Häger, Krister Holmberg

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Citations

Aug 3, 2012·Biomacromolecules·Kai ChenJoseph M DeSimone
Sep 20, 2011·Journal of Photochemistry and Photobiology. B, Biology·Hongxia ChengGuolin Zou
Mar 30, 2010·Journal of Photochemistry and Photobiology. B, Biology·Paulami MandalTapan Ganguly
Oct 27, 2004·Biophysical Chemistry·Wenjun ZhangHugo Scheer
Aug 5, 2003·Journal of Biochemistry and Molecular Biology·Davood Ajloo, Ali A Moosavi-Movahedi
Jan 18, 2006·Bioorganic & Medicinal Chemistry·Luigi MessoriAnna Rita Bilia
Mar 6, 2009·International Journal of Biological Macromolecules·Seyed Jafar MousavyAli Akbar Moosavi-Movahedi
Oct 21, 2006·Biotechnology and Bioengineering·Tetsuji ItohFujio Mizukami
Aug 26, 2009·Archives of Biochemistry and Biophysics·Carlos Guerrero-MendiolaLeticia Ramírez-Silva
Dec 25, 2000·The Journal of Biological Chemistry·P LaschT Grune
Mar 3, 2007·Chembiochem : a European Journal of Chemical Biology·Yoko UrabeKengo Sakaguchi
Sep 24, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Chunhai FanGenxi Li
Oct 29, 2013·Physical Chemistry Chemical Physics : PCCP·Andrea N L BatistaEwan W Blanch
Nov 12, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jianbo LiuQinglin Sheng
Jun 20, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ashok PabbathiAnunay Samanta
Aug 12, 2014·Physical Chemistry Chemical Physics : PCCP·Juliana Angeiras Batista da SilvaRicardo Luiz Longo
Aug 20, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Zhe AnYan Wang
Sep 29, 2020·ACS Applied Materials & Interfaces·Hong ParkSeonki Hong
Jul 12, 2005·Biomacromolecules·Hidetoshi Teramoto, Mitsuhiro Miyazawa

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