Characterization of the subdomains in the N-terminal region of histidine kinase Hik33 in the cyanobacterium Synechocystis sp. PCC 6803

Plant & Cell Physiology
Yohei ShimuraIwane Suzuki

Abstract

Histidine kinase Hik33 responds to a variety of stress conditions and regulates the expression of stress-inducible genes in the cyanobacterium Synechocystis sp. PCC 6803. However, the mechanisms of response and regulation remain unknown. Generally, a histidine kinase perceives a specific signal via its N-terminal region. Hik33 has two transmembrane helices, a periplasmic loop, and HAMP and PAS domains in its N-terminal region, all of which might be involved in signal perception. To investigate the functions of these subdomains in vivo, we expressed a chimeric histidine kinase (Hik33n-SphSc) by fusing the N-terminal region of Hik33 with the C-terminal region of a sensory histidine kinase that is activated under phosphate-deficient conditions, SphS. Hik33n-SphSc responded to several stimuli that are perceived by intact Hik33 and regulated expression of the phoA gene for alkaline phosphatase, which is normally regulated under phosphate-deficient conditions by SphS. We introduced genes for modified versions of Hik33n-SphSc into Synechocystis and monitored expression of phoA under standard and stress conditions. Hik33n-SphSc lacking either the transmembrane helices or both the HAMP and PAS domains had no kinase activity, whereas Hik...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E K MonsonD R Helinski
Dec 31, 1996·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T MizunoS Tabata
Feb 28, 1998·Current Biology : CB·C P Ponting, L Aravind
Jun 28, 2000·Proceedings of the National Academy of Sciences of the United States of America·L Krall, J W Reed
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Apr 12, 2001·Molecular Microbiology·I SuzukiN Murata
Jun 25, 2003·The Journal of Cell Biology·VladimIr ReiserHaruo Saito
Jul 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kay MarinNorio Murata
Dec 23, 2003·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takakazu KanekoSatoshi Tabata
Oct 9, 2004·The Journal of Biological Chemistry·Kalyanee PaithoonrangsaridNorio Murata
May 12, 2007·Molecular Microbiology·Martin Cann
Jul 5, 2007·Methods in Enzymology·Takeshi YoshidaMasayori Inouye
Jun 17, 2008·Cell·Jeffrey M SkerkerMichael T Laub
Oct 24, 2008·Biochemistry·Rebecca A Ayers, Keith Moffat
Dec 27, 2008·Journal of Molecular Biology·Andreas MöglichKeith Moffat
Feb 24, 2009·Protein Expression and Purification·Mariana MartínDiego de Mendoza
Mar 28, 2009·Proceedings. Biological Sciences·Sujith Puthiyaveetil, John F Allen
Aug 14, 2010·Current Biology : CB·Larisa E CybulskiDiego de Mendoza
Jul 19, 2011·Biochimica Et Biophysica Acta·Sofia UnnerståleRoger R Draheim

❮ Previous
Next ❯

Citations

Apr 23, 2013·Photosynthesis Research·Dmitry A LosSuleyman I Allakhverdiev
May 14, 2014·Annual Review of Microbiology·Diego de Mendoza
Sep 10, 2014·International Journal of Molecular Sciences·Nadine FlinnerEnrico Schleiff
Nov 10, 2015·Biochimica Et Biophysica Acta·Annegret Wilde, Yukako Hihara
May 2, 2013·Progress in Lipid Research·Anton I P M de KroonCedric H De Smet
Sep 6, 2014·Plant & Cell Physiology·Yiting ShiShuhua Yang
Jan 6, 2021·Journal of Integrative Plant Biology·Xuexue ChenZhizhong Gong
Oct 15, 2021·Environmental Science and Pollution Research International·Lijun ShenGuang Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.