PMID: 7542446Jul 1, 1995Paper

Characterization of the swelling-induced alkalinization of endocytotic vesicles in fluorescein isothiocyanate-dextran-loaded rat hepatocytes

The Biochemical Journal
R Schreiber, D Häussinger

Abstract

Short-term cultivated rat hepatocytes were allowed to endocytose fluorescein isothiocyanate (FITC)-coupled dextran and the apparent vesicular pH (pHves) was measured by single-cell fluorescence. After 2 h of exposure to FITC-dextran, the apparent pH in the vesicular compartments accessible to endocytosed FITC-dextran was 6.01 +/- 0.05 (n = 39) in normo-osmotic media. Hypo-osmotic exposure increased, whereas hyper-osmotic exposure decreased apparent pHves. by 0.18 +/- 0.02 (n = 26) and 0.12 +/- 0.01 (n = 23) respectively. Incubation of the cells with unlabelled dextran for 2h before a 2-h FITC-dextran exposure had no effect on apparent pHves and its osmosensitivity. When, however, hepatocytes were exposed to unlabelled dextran for 5 h after a 2 h exposure to FITC-dextran, in order to allow transport of endocytosed FITC-dextran to late endocytotic/lysosomal compartments, apparent pHves. decreased to 5.38 +/- 0.04 (n = 12) and the apparent pH in the vesicular compartment containing the dye was no longer sensitive to aniso-osmotic exposure. These findings indicate that the osomosensitivity of pHves. is apparently restricted to early endocytotic compartments. Aniso-osmotic regulation of apparent pHves. in freshly FITC-loaded hepatoc...Continue Reading

Citations

Aug 9, 2002·The Journal of Biological Chemistry·Ann L MooreSteven D Lidofsky
Jun 1, 2006·Acta Physiologica·D HäussingerF Schliess
May 16, 2007·Pflügers Archiv : European journal of physiology·Jae-Seung ParkWon Kyoo Cho
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Jan 31, 2007·Biological Chemistry·Stephan BeckerDieter Häussinger
Jan 1, 1996·Biological Chemistry Hoppe-Seyler·U WarskulatD Haussinger
Feb 1, 1996·European Journal of Biochemistry·J J LuikenA J Meijer
Nov 30, 2007·The Journal of Biological Chemistry·Roland ReinehrDieter Haüssinger
Oct 31, 2002·Analytical Chemistry·Karine AndrieuxCécile Grabielle-Madelmont
Oct 9, 2008·The Journal of Experimental Biology·Khaled H Ahmed, Bernd Pelster
Mar 2, 1999·Biochemical and Biophysical Research Communications·D Häussinger, F Schliess
Aug 6, 1999·Biochimica Et Biophysica Acta·M C LaroccaR A Marinelli
Mar 26, 1999·Gastroenterology·D HäussingerS Vom Dahl

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