Abstract
Short-term cultivated rat hepatocytes were allowed to endocytose fluorescein isothiocyanate (FITC)-coupled dextran and the apparent vesicular pH (pHves) was measured by single-cell fluorescence. After 2 h of exposure to FITC-dextran, the apparent pH in the vesicular compartments accessible to endocytosed FITC-dextran was 6.01 +/- 0.05 (n = 39) in normo-osmotic media. Hypo-osmotic exposure increased, whereas hyper-osmotic exposure decreased apparent pHves. by 0.18 +/- 0.02 (n = 26) and 0.12 +/- 0.01 (n = 23) respectively. Incubation of the cells with unlabelled dextran for 2h before a 2-h FITC-dextran exposure had no effect on apparent pHves and its osmosensitivity. When, however, hepatocytes were exposed to unlabelled dextran for 5 h after a 2 h exposure to FITC-dextran, in order to allow transport of endocytosed FITC-dextran to late endocytotic/lysosomal compartments, apparent pHves. decreased to 5.38 +/- 0.04 (n = 12) and the apparent pH in the vesicular compartment containing the dye was no longer sensitive to aniso-osmotic exposure. These findings indicate that the osomosensitivity of pHves. is apparently restricted to early endocytotic compartments. Aniso-osmotic regulation of apparent pHves. in freshly FITC-loaded hepatoc...Continue Reading
Citations
Aug 1, 1998·Biological Chemistry·S WieseD Häussinger
Dec 10, 1999·The Journal of Clinical Investigation·B A Watts, D W Good
Jun 20, 2001·The Journal of Biological Chemistry·M W RoeS D Lidofsky
Aug 9, 2002·The Journal of Biological Chemistry·Ann L MooreSteven D Lidofsky
Jun 15, 2006·The Journal of Biological Chemistry·Roland ReinehrDieter Haüssinger
Jun 1, 2006·Acta Physiologica·D HäussingerF Schliess
May 16, 2007·Pflügers Archiv : European journal of physiology·Jae-Seung ParkWon Kyoo Cho
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Sep 7, 2000·Pancreas·B HanC Niederau
May 13, 2004·Glia·Freimut SchliessDieter Häussinger
Jan 31, 2007·Biological Chemistry·Stephan BeckerDieter Häussinger
Jan 1, 1996·Biological Chemistry Hoppe-Seyler·U WarskulatD Haussinger
Feb 1, 1996·European Journal of Biochemistry·J J LuikenA J Meijer
Jul 14, 1998·Electrophoresis·I EllingerR Fuchs
Nov 30, 2007·The Journal of Biological Chemistry·Roland ReinehrDieter Haüssinger
Oct 31, 2002·Analytical Chemistry·Karine AndrieuxCécile Grabielle-Madelmont
Oct 9, 2008·The Journal of Experimental Biology·Khaled H Ahmed, Bernd Pelster
Mar 2, 1999·Biochemical and Biophysical Research Communications·D Häussinger, F Schliess
Apr 17, 2002·Cellular Signalling·Maria C LaroccaRaúl A Marinelli
Aug 6, 1999·Biochimica Et Biophysica Acta·M C LaroccaR A Marinelli
Mar 26, 1999·Gastroenterology·D HäussingerS Vom Dahl