Characterization of the Symbiotic Nitrogen-Fixing Common Bean Low Phytic Acid (lpa1) Mutant Response to Water Stress

Genes
Remo ChiozzottoGeorgina Hernández

Abstract

The common bean (Phaseolus vulgarisL.)low phytic acid(lpa1) biofortified genotype produces seeds with improved nutritional characteristics and does not display negative pleiotropic effects. Here we demonstrated thatlpa1plants establish an efficient nitrogen-fixing symbiosis withRhizobium etliCE3. Thelpa1nodules showed a higher expression of nodule-function related genes than the nodules of the parental wild type genotype (BAT 93). We analyzed the response to water stress oflpa1vs. BAT 93 plants grown under fertilized or under symbiotic N₂-fixation conditions. Water stress was induced by water withholding (up to 14% soil moisture) to fertilized orR. etlinodulated plants previously grown with normal irrigation. The fertilizedlpa1plants showed milder water stress symptoms during the water deployment period and after the rehydration recovery period whenlpa1plants showed less biomass reduction. The symbiotic water-stressedlpa1plants showed decreased nitrogenase activity that coincides with decreased sucrose synthase gene expression in nodules; lower turgor weight to dry weight (DW) ratio, which has been associated with higher drought resistance index; downregulation of carbon/nitrogen (C/N)-related and upregulation of stress-related...Continue Reading

References

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Methods Mentioned

BETA
PCR

Software Mentioned

7300 Real - Time PCR
7300 System
HarvestPlus

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