Abstract
We isolated Comamonas sp. strain E6, which utilizes terephthalate (TPA) as the sole carbon and energy source via the protocatechuate (PCA) 4,5-cleavage pathway. Two almost identical TPA degradation gene clusters, tphRICIA2IA3IBIA1I and tphRIICIIA2IIA3IIBIIA1II, were isolated from this strain. Based on amino acid sequence similarity, the genes tphR, tphC, tphA2, tphA3, tphB, and tphA1 were predicted to code, respectively, for an IclR-type transcriptional regulator, a periplasmic TPA binding receptor, the large subunit of the oxygenase component of TPA 1,2-dioxygenase (TPADO), the small subunit of the oxygenase component of TPADO, a 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate (DCD) dehydrogenase, and a reductase component of TPADO. The growth of E6 on TPA was not affected by disruption of either tphA2I or tphA2II singly; however, the tphA2I tphA2II double mutant no longer grew on TPA, suggesting that both TPADO genes are involved in TPA degradation. Introduction of a plasmid carrying tphRIICIIA2IIA3IIBIIA1II conferred the TPA utilization phenotype on Comamonas testosteroni IAM 1152, which is able to grow on PCA but not on TPA. Disruption of either tphRII or tphCII on this plasmid resulted in the loss of the growth of IAM 1...Continue Reading
References
Mar 1, 1979·Applied and Environmental Microbiology·R L CrawfordP E Perkins-Olson
May 7, 1976·Analytical Biochemistry·M M Bradford
Jan 1, 1990·Journal of Bacteriology·B E Haigler, D T Gibson
Jan 1, 1990·Journal of Bacteriology·B E Haigler, D T Gibson
Aug 11, 1988·Nucleic Acids Research·J M ShortW D Huse
Jan 1, 1985·Gene·C Yanisch-PerronJ Messing
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I Saito, G R Stark
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Dec 1, 1981·Gene·M BagdasarianK N Timmis
Oct 1, 1982·Gene·J Vieira, J Messing
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Feb 1, 1983·Journal of Biochemistry·K Maruyama
Dec 1, 1982·Journal of Bacteriology·P J KerstenJ D Lipscomb
Nov 1, 1994·Journal of Bacteriology·H R SchläfliA M Cook
Jul 22, 1994·Gene·A SchäferA Pühler
Jun 1, 1995·Environmental Health Perspectives·Y Z WangG J Zylstra
Jan 1, 1996·Annual Review of Microbiology·C S Harwood, R E Parales
Jan 1, 1997·Plasmid·J M BlatnyS Valla
Sep 19, 1997·Journal of Bacteriology·L S CollierE L Neidle
Mar 12, 1998·Journal of Bacteriology·D EulbergM Schlömann
Mar 21, 1998·Applied and Environmental Microbiology·S NishikawaY Katayama
Apr 18, 1998·The Journal of Biological Chemistry·H ItzhakiZ Adam
Jun 23, 1998·Structure·B KauppiS Ramaswamy
Jul 2, 1998·Applied and Environmental Microbiology·X PengM Fukuda
Dec 29, 1998·Journal of Bacteriology·E MasaiM Fukuda
Feb 27, 1999·Journal of Bacteriology·M F RomineJ D Saffer
Nov 14, 2000·Journal of Bacteriology·E MasaiM Fukuda
Nov 28, 2000·Journal of Bacteriology·H HaraM Fukuda
Dec 18, 2002·Journal of Bacteriology·Hirofumi HaraMasao Fukuda
Feb 4, 2003·Journal of Bacteriology·Rudy AntoineCamille Locht
Apr 3, 2003·FEMS Microbiology Letters·Toru ShigematsuKenji Kida
Sep 23, 2003·Research in Microbiology·Brit WinnenMilton H Saier
Mar 4, 2005·Journal of Bacteriology·Tomokuni AbeMasao Fukuda
Sep 27, 2005·FEMS Microbiology Letters·Ki Young ChoiEungbin Kim
Citations
Mar 1, 2008·Indian Journal of Microbiology·C Vamsee-Krishna, Prashant S Phale
Jul 27, 2010·Applied and Environmental Microbiology·Daisuke KasaiEiji Masai
Nov 26, 2009·Applied and Environmental Microbiology·Yuki FukuharaEiji Masai
Oct 19, 2010·Applied and Environmental Microbiology·Naofumi KamimuraEiji Masai
Sep 1, 2009·Journal of Bacteriology·Daisuke KasaiEiji Masai
Dec 5, 2006·Journal of Bacteriology·Hirofumi HaraWilliam W Mohn
Aug 31, 2013·Standards in Genomic Sciences·Michael WeissDavid Schleheck
Apr 30, 2013·Applied Microbiology and Biotechnology·Ti Fang, Ning-Yi Zhou
Feb 11, 2011·Bioscience, Biotechnology, and Biochemistry·Akio TaniFusako Kawai
Sep 9, 2008·Bioscience, Biotechnology, and Biochemistry·Yuki FukuharaEiji Masai
Dec 7, 2011·Enzyme and Microbial Technology·Shunsuke ImaiMasao Fukuda
Jan 3, 2016·Journal of Bioscience and Bioengineering·Yuriko Okamura-AbeYoshihiro Katayama
Mar 12, 2016·Science·Shosuke YoshidaKohei Oda
Oct 27, 2011·Environmental Microbiology·Danilo Pérez-PantojaBernardo González
Aug 15, 2012·Journal of Molecular Biology·Jasleen BainsMartin J Boulanger
May 26, 2015·Journal of Hazardous Materials·Sailas BenjaminEiji Masai
Aug 5, 2009·The Journal of Veterinary Medical Science·Mohamed Mahdi AlshahniTakuo Sawada
Jun 20, 2015·Genome Announcements·Jun ShimodairaEiji Masai
Feb 9, 2012·FEMS Microbiology Letters·Naofumi KamimuraEiji Masai
Jun 2, 2018·Letters in Applied Microbiology·J LiuM Jiang
Aug 6, 2013·Applied and Environmental Microbiology·Masaru HosakaEiji Masai
May 19, 2019·Genes·Manuel SalvadorJose I Jimenez
Feb 29, 2020·Microorganisms·Michael Onwona-KwakyeCharles Darkoh
May 3, 2020·Genome Biology and Evolution·Başak ÖztürkDirk Springael
Dec 20, 2015·Applied and Environmental Microbiology·Kai ChenJiandong Jiang
Mar 10, 2017·Microbial Biotechnology·Agnieszka GawinTrygve Brautaset
Jan 1, 2016·3 Biotech·Vinay Kumar, S S Maitra
Feb 6, 2019·Scientific Reports·Daisuke KasaiMasao Fukuda
Sep 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Brandon C KnottJohn E McGeehan
May 7, 2020·Frontiers in Microbiology·Jiakang RuYu Yang
Apr 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Harry P AustinGregg T Beckham
Jan 7, 2021·Microbial Biotechnology·Tanja NarancicJosé I Jiménez
Nov 15, 2020·Nature Communications·Ingrid E Meyer-CifuentesBaşak Öztürk
Jan 17, 2021·Applied and Environmental Microbiology·Yan-Jie HouNing-Yi Zhou
May 21, 2021·Biotechnology Advances·Octavio García-DepraectRaúl Muñoz
Jun 15, 2021·Frontiers in Bioengineering and Biotechnology·Lakshika Dissanayake, Lahiru N Jayakody
Jul 20, 2021·Green Chemistry : an International Journal and Green Chemistry Resource : GC·Joanna C Sadler, Stephen Wallace
Sep 27, 2020·Metabolic Engineering·Isabel PardoChristopher W Johnson
Jul 16, 2021·Metabolic Engineering·Allison Z WernerGregg T Beckham
Oct 31, 2021·Nature Communications·Trishnamoni GautomNeil Dixon