Characterization of the terephthalate degradation genes of Comamonas sp. strain E6

Applied and Environmental Microbiology
Mikio SasohMasao Fukuda

Abstract

We isolated Comamonas sp. strain E6, which utilizes terephthalate (TPA) as the sole carbon and energy source via the protocatechuate (PCA) 4,5-cleavage pathway. Two almost identical TPA degradation gene clusters, tphRICIA2IA3IBIA1I and tphRIICIIA2IIA3IIBIIA1II, were isolated from this strain. Based on amino acid sequence similarity, the genes tphR, tphC, tphA2, tphA3, tphB, and tphA1 were predicted to code, respectively, for an IclR-type transcriptional regulator, a periplasmic TPA binding receptor, the large subunit of the oxygenase component of TPA 1,2-dioxygenase (TPADO), the small subunit of the oxygenase component of TPADO, a 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate (DCD) dehydrogenase, and a reductase component of TPADO. The growth of E6 on TPA was not affected by disruption of either tphA2I or tphA2II singly; however, the tphA2I tphA2II double mutant no longer grew on TPA, suggesting that both TPADO genes are involved in TPA degradation. Introduction of a plasmid carrying tphRIICIIA2IIA3IIBIIA1II conferred the TPA utilization phenotype on Comamonas testosteroni IAM 1152, which is able to grow on PCA but not on TPA. Disruption of either tphRII or tphCII on this plasmid resulted in the loss of the growth of IAM 1...Continue Reading

References

Mar 1, 1979·Applied and Environmental Microbiology·R L CrawfordP E Perkins-Olson
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I Saito, G R Stark
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Feb 1, 1983·Journal of Biochemistry·K Maruyama
Dec 1, 1982·Journal of Bacteriology·P J KerstenJ D Lipscomb
Jun 1, 1995·Environmental Health Perspectives·Y Z WangG J Zylstra
Jan 1, 1996·Annual Review of Microbiology·C S Harwood, R E Parales
Feb 4, 2003·Journal of Bacteriology·Rudy AntoineCamille Locht
Sep 23, 2003·Research in Microbiology·Brit WinnenMilton H Saier

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Citations

Mar 1, 2008·Indian Journal of Microbiology·C Vamsee-Krishna, Prashant S Phale
Jul 27, 2010·Applied and Environmental Microbiology·Daisuke KasaiEiji Masai
Nov 26, 2009·Applied and Environmental Microbiology·Yuki FukuharaEiji Masai
Sep 1, 2009·Journal of Bacteriology·Daisuke KasaiEiji Masai
Aug 31, 2013·Standards in Genomic Sciences·Michael WeissDavid Schleheck
Sep 9, 2008·Bioscience, Biotechnology, and Biochemistry·Yuki FukuharaEiji Masai
Mar 12, 2016·Science·Shosuke YoshidaKohei Oda
Oct 27, 2011·Environmental Microbiology·Danilo Pérez-PantojaBernardo González
May 26, 2015·Journal of Hazardous Materials·Sailas BenjaminEiji Masai
Aug 5, 2009·The Journal of Veterinary Medical Science·Mohamed Mahdi AlshahniTakuo Sawada
Feb 9, 2012·FEMS Microbiology Letters·Naofumi KamimuraEiji Masai
Aug 6, 2013·Applied and Environmental Microbiology·Masaru HosakaEiji Masai
May 19, 2019·Genes·Manuel SalvadorJose I Jimenez
Feb 29, 2020·Microorganisms·Michael Onwona-KwakyeCharles Darkoh
Dec 20, 2015·Applied and Environmental Microbiology·Kai ChenJiandong Jiang
Sep 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Brandon C KnottJohn E McGeehan
May 7, 2020·Frontiers in Microbiology·Jiakang RuYu Yang
Apr 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Harry P AustinGregg T Beckham
Nov 15, 2020·Nature Communications·Ingrid E Meyer-CifuentesBaşak Öztürk
Jun 15, 2021·Frontiers in Bioengineering and Biotechnology·Lakshika Dissanayake, Lahiru N Jayakody
Jul 20, 2021·Green Chemistry : an International Journal and Green Chemistry Resource : GC·Joanna C Sadler, Stephen Wallace
Oct 31, 2021·Nature Communications·Trishnamoni GautomNeil Dixon

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