Characterization of the TPX2 domains involved in microtubule nucleation and spindle assembly in Xenopus egg extracts

Molecular Biology of the Cell
Stéphane BrunetIsabelle Vernos

Abstract

TPX2 has multiple functions during mitosis, including microtubule nucleation around the chromosomes and the targeting of Xklp2 and Aurora A to the spindle. We have performed a detailed domain functional analysis of TPX2 and found that a large N-terminal domain containing the Aurora A binding peptide interacts directly with and nucleates microtubules in pure tubulin solutions. However, it cannot substitute the endogenous TPX2 to support microtubule nucleation in response to Ran guanosine triphosphate (GTP) and spindle assembly in egg extracts. By contrast, a large C-terminal domain of TPX2 that does not bind directly to pure microtubules and does not bind Aurora A kinase rescues microtubule nucleation in response to RanGTP and spindle assembly in TPX2-depleted extract. These and previous results suggest that under physiological conditions, TPX2 is essential for microtubule nucleation around chromatin and functions in a network of other molecules, some of which also are regulated by RanGTP.

References

Mar 1, 1991·The Journal of Cell Biology·K E Sawin, T J Mitchison
Jan 1, 1991·Methods in Enzymology·A HymanT Mitchison
Nov 22, 1997·Current Biology : CB·K E Sawin, P Nurse
Nov 22, 1997·Current Biology : CB·M B ElowitzS Leibler
Jan 19, 1999·Methods in Cell Biology·M Bornens, M Moudjou
Dec 22, 1999·Trends in Cell Biology·R Heald, K Weis
Jun 28, 2000·The Journal of Cell Biology·T WittmannI Vernos
Mar 17, 2001·Science·J A Kahana, D W Cleveland
Oct 20, 2001·Science·E Karsenti, I Vernos
Aug 15, 2002·Current Biology : CB·Mary Dasso
Aug 15, 2002·The Journal of Cell Biology·Thomas A KuferErich A Nigg
Feb 11, 2003·Nature Cell Biology·Ming-Ying TsaiYixian Zheng
Apr 18, 2003·Current Biology : CB·Patrick A EyersJames L Maller
May 3, 2003·The EMBO Journal·Christoph A SchatzRafael E Carazo-Salas
Oct 29, 2003·Molecular Cell·Richard BaylissElena Conti

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Citations

Aug 4, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Susan Doubilet, Kim S McKim
Mar 26, 2010·The Journal of Biological Chemistry·Astrid Giesecke, Murray Stewart
Sep 19, 2008·Molecular Biology of the Cell·Claudia M CasanovaIain W Mattaj
Jan 30, 2010·Molecular Biology of the Cell·Nan MaPatricia Wadsworth
Oct 23, 2008·The Plant Cell·Jan W VosAnne-Catherine Schmit
Mar 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maïa ChanrionJean-Marie Darbon
Nov 7, 2009·Disease Models & Mechanisms·Marie K Cross, Maureen A Powers
Dec 15, 2010·Journal of Cell Science·Maria GiubettiniGiulia Guarguaglini
Oct 4, 2008·PloS One·Stéphane BrunetMarie-Hélène Verlhac
Jan 1, 2008·Advances and Applications in Bioinformatics and Chemistry : AABC·Alex S RajangamLars Arvestad
Feb 22, 2014·Cellular and Molecular Life Sciences : CMLS·Gernot NeumayerMinh Dang Nguyen
Jul 30, 2014·The Journal of Cell Biology·Kara J Helmke, Rebecca Heald
Jul 30, 2008·The Journal of Cell Biology·Alexander W Bird, Anthony A Hyman
Aug 5, 2015·The Journal of Cell Biology·Jingyan FuChuanmao Zhang
Dec 24, 2014·Current Biology : CB·Jacopo ScrofaniIsabelle Vernos
Dec 5, 2015·Frontiers in Cell and Developmental Biology·Jack W C ChenJames G Wakefield
Aug 30, 2008·The EMBO Journal·Teresa SardonIsabelle Vernos
Feb 27, 2007·The Plant Journal : for Cell and Molecular Biology·Robyn M PerrinPatrick H Masson
Mar 26, 2013·The Plant Journal : for Cell and Molecular Biology·Kinda MasoudAnne-Catherine Schmit
Aug 11, 2015·Cytoskeleton·Olga I KahnPeter W Baas
Jun 23, 2015·Nature Cell Biology·Michal WieczorekGary J Brouhard
Aug 26, 2009·Plant Signaling & Behavior·Jean-Luc EvrardAnne-Catherine Schmit
Mar 29, 2008·Current Biology : CB·Frank EckerdtJames L Maller
Aug 20, 2005·Molecular Cell·Eric Karsenti
Oct 21, 2014·PloS One·Agnieszka Kaczmarczyk, Kevin F Sullivan
Oct 27, 2016·BMC Structural Biology·Luis Sanchez-PulidoMiguel A Andrade-Navarro
Nov 18, 2016·Molecular Biology of the Cell·Barbara J MannPatricia Wadsworth
Jan 18, 2017·Genesis : the Journal of Genetics and Development·Georgina A Stooke-VaughanSarah Woolner
Mar 8, 2017·The Journal of Cell Biology·Raymundo Alfaro-AcoSabine Petry
Nov 10, 2017·ELife·Rui ZhangEva Nogales
Aug 24, 2017·Nature Reviews. Molecular Cell Biology·Johanna Roostalu, Thomas Surrey

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