Characterization of the transcriptional activators SalA and SyrF, Which are required for syringomycin and syringopeptin production by Pseudomonas syringae pv. syringae.

Journal of Bacteriology
Nian WangDennis C Gross

Abstract

Production of the phytotoxins syringomycin and syringopeptin by Pseudomonas syringae pv. syringae is controlled by the regulatory genes salA and syrF. Analysis with 70-mer oligonucleotide microarrays established that the syr-syp genes responsible for synthesis and secretion of syringomycin and syringopeptin belong to the SyrF regulon. Vector pMEKm12 was successfully used to express both SalA and SyrF proteins fused to a maltose-binding protein (MBP) in Escherichia coli and P. syringae pv. syringae. Both the MBP-SalA and MBP-SyrF fusion proteins were purified by maltose affinity chromatography. Gel shift analysis revealed that the purified MBP-SyrF, but not the MBP-SalA fusion protein, bound to a 262-bp fragment of the syrB1 promoter region containing the syr-syp box. Purified MBP-SalA caused a shift of a 324-bp band containing the putative syrF promoter. Gel filtration analysis and cross-linking experiments indicated that both SalA and SyrF form homodimers in vitro. Overexpression of the N-terminal regions of SalA and SyrF resulted in decreased syringomycin production by strain B301D and reduced levels of beta-glucuronidase activities of the sypA::uidA and syrB1::uidA reporters by 59% to 74%. The effect of SalA on the expressio...Continue Reading

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Citations

Mar 1, 2012·The Journal of Microbiology·Hyosun Cho, Hyojeung Kang
Aug 3, 2012·Molecular Plant-microbe Interactions : MPMI·Christina RamelRobert Dudler
Dec 31, 2008·Journal of Bacteriology·I de Bruijn, J M Raaijmakers
Nov 13, 2007·Journal of Bacteriology·I de BruijnJ M Raaijmakers
Feb 23, 2011·International Journal of Molecular Sciences·Niran RoongsawangMasaaki Morikawa
Apr 12, 2014·Genome Announcements·Alexey Dudnik, Robert Dudler
Jun 1, 2010·Environmental Microbiology Reports·Jolien D'aesMonica Höfte
Apr 24, 2010·FEMS Microbiology Reviews·Jos M RaaijmakersMarc Ongena
Sep 10, 2014·FEMS Microbiology Letters·Chunxu SongJos M Raaijmakers
Aug 23, 2019·Natural Product Reports·Sebastian Götze, Pierre Stallforth
Sep 5, 2020·Critical Reviews in Microbiology·Léa GirardRené De Mot

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