Characterization of the transcriptional regulation of the regulator of G protein signaling 2 (RGS2) gene during 3T3-L1 preadipocyte differentiation

Journal of Cellular Biochemistry
Ya-Shan ChengYuh-Lin Wu

Abstract

Adipocyte differentiation is a complex process involving several signaling pathways. Molecular mechanisms regulating the very early stage of adipocyte differentiation is not fully appreciated yet. Several inducible genes at the early stage of preadipocyte differentiation have been identified, including the regulator of G protein signaling 2 (RGS2), a member of the RGS protein superfamily. This study aimed to clarify the precise induction profile of RGS2 and to determine the essential transcription element(s) regulating RGS2 expression in differentiating 3T3-L1 preadipocytes. RGS2 mRNA expression was elevated immediately at 1 h after differentiation initiation and it remained high until the late stage of differentiation. The putative promoter sequence (approximately 3,000 bp) of the mouse RGS2 gene was isolated and the RGS2 promoter activity was significantly upregulated 3 h after inducing differentiation. The primary signaling pathway leading to RGS2 transcriptional activation appeared to be cAMP-dependent. Sequential deletion and site-directed mutagenesis strategies demonstrate that the RGS2 promoter sequence truncated down to 78 bp in size retained full inducibility by the differentiation stimuli. Mutation of a Sp1 site withi...Continue Reading

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Citations

Oct 27, 2010·Journal of Molecular Cell Biology·Zhihui XieKirk M Druey
May 1, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Jacob KachNickolai O Dulin
Dec 29, 2009·Frontiers in Bioscience (Elite Edition)·Zhenyu JiXiaodong Cheng
Aug 28, 2012·Biochemical and Biophysical Research Communications·Chau H NguyenPeter Chidiac

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