PMID: 2110058Mar 30, 1990Paper

Characterization of the translational start site for IF2 beta, a short form of Escherichia coli initiation factor IF2

European Journal of Biochemistry
F Morel-DevilleY Cenatiempo

Abstract

The gene for initiation factor IF2, infB, represents one of the few examples in Escherichia coli of genes encoding two protein products in vivo. In a previous work, our group showed that both forms of IF2 (alpha and beta) are closely related and may arise from two independent translational events on infB mRNA. Unambiguous mapping and rigorous determination of the nature of the initiation triplet for IF2 beta, the smaller form of IF2, is critical for future mutagenesis of this codon, required for investigating the biological importance of both IF2 alpha and IF2 beta. Three types of experiments were carried out. First, a 77-bp deletion was created at the beginning of the structural gene leading to premature termination of IF2 alpha synthesis. Under these conditions, IF2 beta is still formed. Second, various Bal31 digests of infB containing the 77-bp deletion were fused to lacZ. Any synthesis of a fused protein with beta-galactosidase activity should reflect the occurrence of an initiation event on the messenger corresponding to this DNA segment. It was consequently possible to locate the IF2 beta initiation site within an 18-base region containing an in-phase GUG codon. Third, to avoid any artefactual reinitiation event possibly ...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·F Bolivar, K Backman
Jan 1, 1975·Current Topics in Microbiology and Immunology·A C Frazer, R Curtiss
Jan 1, 1988·Gene·F BleicherY Cenatiempo
Jul 3, 1987·Cell·W J Craigen, C T Caskey
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J G FilesJ H Miller
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C SacerdotM Grunberg-Manago
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J A PlumbridgeM Grunberg-Manago
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Jul 10, 1981·Nucleic Acids Research·D Ish-Horowicz, J F Burke
Jan 1, 1983·Molecular & General Genetics : MGG·T Kurihara, Y Nakamura
Jan 1, 1984·Molecular & General Genetics : MGG·E T Palva, T J Silhavy
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S IshiiF Imamoto
Aug 25, 1984·Journal of Molecular Biology·L M MunsonW S Reznikoff
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R A Smith, J S Parkinson
May 11, 1982·Nucleic Acids Research·G D StormoL M Gold
Jul 5, 1981·Journal of Molecular Biology·C NapoliB S Singer
Feb 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·H I AdlerA A Hardigree

❮ Previous
Next ❯

Citations

Jul 20, 1991·Journal of Molecular Biology·S LaalamiM Grunberg-Manago
Dec 1, 1991·Biochimie·S LaalamiM Grunberg-Manago
Jan 15, 1997·European Journal of Biochemistry·H P VornlocherM Sprinzl
Apr 9, 2008·Molekuliarnaia biologiia·A R KaiumovM R Sharipova
Dec 31, 1991·Biochemical and Biophysical Research Communications·N R NyengaardH U Sperling-Petersen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.