Characterization of the Treponema denticola prtP gene encoding a prolyl-phenylalanine-specific protease (dentilisin).

Infection and Immunity
Kazuyuki IshiharaKatsuji Okuda

Abstract

A chymotrypsin-like protease from Treponema denticola ATCC 35405 was purified by chromatographic techniques. The purified enzyme consisted of three polypeptides (38, 43, and 72 kDa). The protease exhibited specificity for peptide bonds containing phenylalanine and proline at the P1 and P2 positions, respectively, and was classified as a serine protease on the basis of inhibition studies. Naturally occurring protease inhibitors such as alpha1-antitrypsin and alpha1-antichymotrypsin had no effect on enzymatic activity. The enzyme degraded fibronectin, alpha1-antitrypsin, and gelatin while weakly degrading the immunoglobulin G heavy chain and type IV collagen. N-terminal amino acid sequences were determined for the 43- and 72-kDa proteins. On the basis of these sequences, the genes coding for the 43- and 72-kDa proteins were isolated and sequenced. The open reading frame which codes for the 72-kDa protein was designated prtP. This gene consists of 2,169 bp and codes for a protein with an Mr of 77,471. The protein appeared to be composed of a signal peptide region followed by a prosequence and the mature protein domain. The deduced amino acid sequence exhibited similarity with that of the Bacillus subtilis serine protease subtilisi...Continue Reading

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Citations

Sep 10, 1999·Microbiology and Immunology·K Ishihara, K Okuda
Nov 24, 1999·Journal of Periodontal Research·G RosenM N Sela
Dec 10, 1999·FEMS Microbiology Letters·K Ishihara, K Okuda
Jul 17, 2004·Microbiology·Peter M Vesey, Howard K Kuramitsu
Apr 12, 2005·BMC Infectious Diseases·Serguei G PopovKen Alibek
May 19, 2001·Oral Microbiology and Immunology·Q D DengH K Kuramitsu
Jul 24, 2004·Infection and Immunity·Akihiko IkegamiHoward K Kuramitsu
Feb 19, 2008·Oral Microbiology and Immunology·R CaponeJ C Fenno
Feb 28, 2007·Infection and Immunity·Catherine A Brissette, Sheila A Lukehart
Nov 18, 2003·Journal of Bacteriology·Frederick F CorreiaFloyd E Dewhirst
Apr 28, 2005·Periodontology 2000·Richard P Ellen, Vaia B Galimanas
Aug 18, 2010·Periodontology 2000·Kazuyuki Ishihara
May 4, 2010·Journal of Bacteriology·Valentina GodovikovaJ Christopher Fenno
Oct 14, 2010·Journal of Dental Research·S G DashperE C Reynolds
Feb 14, 2014·The Bulletin of Tokyo Dental College·Isato YamamotoKatsuji Okuda
Sep 29, 2004·FEMS Microbiology Reviews·Paul A CullenBen Adler
Oct 12, 2011·Infection and Immunity·Valentina GodovikovaJ Christopher Fenno
Dec 15, 2010·Infection and Immunity·Simon HoustonCaroline E Cameron
Apr 27, 2004·Periodontology 2000·Neil M O'Brien-SimpsonEric C Reynolds
May 21, 2003·Clinical Oral Implants Research·Michael N SelaGraciela Rosen
Jan 1, 1997·Microbiology and Immunology·T UmemotoI Namikawa
Mar 23, 2004·FEMS Microbiology Letters·Kazuyuki IshiharaKatsuji Okuda

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