PMID: 2110653Apr 11, 1990Paper

Characterization of the v-myb DNA binding domain

Nucleic Acids Research
T OehlerKarl-Heinz Klempnauer

Abstract

The transforming protein encoded by the v-myb oncogene is a sequence-specific DNA-binding protein that is thought to be involved in the regulation of gene expression. The N-terminal region of the v-myb protein is composed of two highly conserved tandem repeat sequences of unknown function. It has been speculated that the N-terminal v-myb repeats might be crucial for DNA-binding, since N-terminal deletions destroy the DNA-binding activity of the v-myb protein. Here, we have studied the v-myb DNA-binding domain in more detail. Our results show that the N-terminal region of the v-myb protein is sufficient for specific DNA-binding. Dissection of this region suggests that both repeats are required for DNA-binding, but that both repeats play different roles in v-myb protein DNA interaction. We also show that the myb repeats of a drosophila melanogaster homolog of c-myb function as sequence-specific DNA-binding domain. Our results support the view that specific sequence-recognition, mediated by the conserved myb repeats, is a general feature of myb-related proteins.

References

Oct 1, 1989·Genes & Development·K H KlempnauerH Biedenkapp
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T P Bender, W M Kuehl
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y WeinsteinE P Reddy
Oct 27, 1988·Nature·H BiedenkappK H Klempnauer
Jun 1, 1985·Cell·A L KatzenJ M Bishop
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M G Moscovici, C Moscovici
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W J BoyleM A Baluda
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E H WestinF Wong-Staal

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Citations

Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P SaikumarE P Reddy
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E KerkhoffK H Klempnauer
Jul 25, 1991·Nucleic Acids Research·K M Howe, R J Watson
Apr 11, 1991·Nucleic Acids Research·D M Bortner, M C Ostrowski
Sep 10, 2004·Nucleic Acids Research·Daniel BraasKarl-Heinz Klempnauer
Dec 17, 2004·Molecular and Cellular Biology·Olesya ChaykaKarl-Heinz Klempnauer
Sep 8, 2007·BMC Molecular Biology·Holger GundelachKarl-Heinz Klempnauer
Aug 14, 2001·Blood Cells, Molecules & Diseases·J JacksonA L Katzen
Feb 20, 1998·Biophysical Chemistry·R V HosurL C Padhy
Dec 1, 1993·Molecular and Cellular Biology·P W Dini, J S Lipsick
Apr 1, 1996·Molecular and Cellular Biology·S MinkK H Klempnauer

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