Characterization of the varicella zoster virus (VZV)-specific intra-ocular T-cell response in patients with VZV-induced uveitis

Experimental Eye Research
Johannes C MilikanGeorges M G M Verjans

Abstract

Varicella zoster virus (VZV) is a well-known cause of infectious uveitis. The aim of this study was to characterize the VZV-specific T-cell response in eyes of patients with VZV-induced uveitis. T-cell lines (TCL) were generated by mitogenic stimulation of intra-ocular fluid (IOF) samples obtained from eight patients with VZV-induced uveitis. Two patients with herpes simplex virus (HSV)-induced uveitis were included as disease controls. Characterization of individual T-cells in the TCL was performed by stimulating the TCL with mock, HSV-1 and VZV antigen pulsed autologous B cells and subsequent flow cytometric analyses. Virus specificity and phenotype of the T-cells were identified by simultaneous detection of intracellular gamma interferon and cell surface markers CD4, CD8, CD3 or T-cell receptor (TCR) beta chain variable region (TCRBV) usage. The TCL obtained from patients with HSV-1-induced uveitis contained higher numbers of T-cells reactive to HSV-1 compared to VZV. VZV-specific T-cells were detected in all TCL of the patients diagnosed with VZV-induced uveitis. Four out of six TCL obtained from patients with VZV-induced uveitis that were assayed for both viruses, contained higher numbers of T-cells reactive to VZV compare...Continue Reading

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Citations

Apr 12, 2012·The Journal of Infectious Diseases·Saskia L SmitsGeorges M G M Verjans
Jul 30, 2015·Ocular Immunology and Inflammation·Elke Olivia KrepsPhilippe Kestelyn
Aug 14, 2010·Ophthalmology·Toine HillenaarLies Remeijer
May 15, 2013·Current Opinion in Virology·Werner J D OuwendijkDavid M Koelle
Mar 14, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Werner J D OuwendijkGeorges M G M Verjans
Oct 27, 2006·Current Opinion in Ophthalmology
Jun 15, 2007·Current Opinion in Ophthalmology

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