Abstract
From the venom of Chinese green tree viper (Trimeresurus stejnegeri), three distinct fibrinogenolytic enzymes: stejnefibrase-1, stejnefibrase-2 and stejnefibrase-3, were purified by gel filtration, ion-exchange chromatography and reverse-phase high-performance chromatography (HPLC). SDS-PAGE analysis of those three enzymes showed that they consisted of a single polypeptide chain with mol. wt of 50000, 31000 and 32000, respectively. Like TSV-PA (a specific plasminogen activator) and stejnobin (a fibrinogen-clotting enzyme) purified from the same venom, stejnefibrase-1, -2 and -3 were able to hydrolyze several chromogenic substrate. On the other hand, different from TSV-PA and stejnobin, stejnefibrase-1, -2 and -3 did not activate plasminogen and did not possess fibrinogen-clotting activity. The three purified enzymes directly degraded fibrinogen to small fragments and rendered it unclottable by thrombin. Stejnefibrase-2 degraded preferentially Bbeta-chain while stejnefibrase-1 and -3 cleaved concomitantly Aalpha and Bbeta-chains of fibrinogen. None of these proteases degraded the gamma-chain of fibrinogen. When correlated with the loss of clottability of fibrinogen, the most active enzyme was stejnefibrase-1. The activities of t...Continue Reading
References
Dec 7, 1979·Biochimica Et Biophysica Acta·C Ouyang, T F Huang
Aug 8, 1978·Biochemistry·J B Bjarnason, A T Tu
Sep 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·C OuyangT F Huang
Aug 1, 1991·Thrombosis and Haemostasis·H Pirkle, N Marsh
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·E SiigurJ Siigur
Jan 1, 1991·Critical Reviews in Toxicology·J Meier, K Stocker
Feb 10, 1987·Biochemistry·H Hofmann, C Bon
Aug 15, 1970·Nature·U K Laemmli
May 14, 1981·Biochimica Et Biophysica Acta·J B Moran, C R Geren
Feb 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·K Shimokawa, H Takahashi
Apr 28, 1995·The Journal of Biological Chemistry·Y ZhangC Bon
Jul 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·M T AssakuraF R Mandelbaum
May 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·Y ZhangS W Zu
Aug 15, 1997·The Journal of Biological Chemistry·Y ZhangC Bon
May 30, 1998·Toxicon : Official Journal of the International Society on Toxinology·Y ZhangW Y Wang
Citations
Apr 18, 2001·Toxicon : Official Journal of the International Society on Toxinology·Y JinY L Xiong
Aug 15, 2002·Toxicon : Official Journal of the International Society on Toxinology·D K JagadeeshaK Kemparaju
Jul 24, 2012·Biochemistry. Biokhimii︠a︡·Yashonandana J GowthamK Kemparaju
Mar 20, 2010·PloS One·Sakthivel VaiyapuriGail Hutchinson
May 8, 2010·Zoological Research·Qiu-Min LuYun Zhang
Mar 24, 2009·Protein Expression and Purification·Yixin LinJiangyu Deng
Jul 16, 2008·Toxicon : Official Journal of the International Society on Toxinology·Rong GaoPonnampalam Gopalakrishnakone
Mar 10, 2007·Toxicon : Official Journal of the International Society on Toxinology·Chuanchom Muanpasitporn, Ponlapat Rojnuckarin
Aug 30, 2008·Journal of Biochemical and Molecular Toxicology·Suphan SoogarunTusrin Moungkote
Jul 11, 2012·The Journal of Pharmacy and Pharmacology·Martin Flemmig, Matthias F Melzig
May 7, 2014·Biotechnology Progress·Essam Kotb
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Yang JinYu-Liang Xiong
Dec 7, 2005·Toxicon : Official Journal of the International Society on Toxinology·Shu-Rong ZhongYun Zhang
Aug 22, 2012·Journal of Proteomics·Mauren VillaltaJosé María Gutiérrez
Jul 24, 2012·Biochimie·Danilo Luccas MenaldoSuely Vilela Sampaio
Dec 14, 2011·Toxicon : Official Journal of the International Society on Toxinology·José Antonio Valeriano-ZapanaSergio Marangoni
Jun 22, 2016·Journal of Proteomics·Zorica LatinovićIgor Križaj
Aug 11, 2004·Toxicon : Official Journal of the International Society on Toxinology·Run-Qiang ChenYu-Liang Xiong
Oct 30, 2016·Bioengineered· Adivitiya, Yogender Pal Khasa
May 31, 2017·Journal of Proteomics·Zhang-Min YangInn-Ho Tsai
Dec 20, 2018·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Ângela Alice Amadeu MegaleWilmar Dias da Silva
Jan 27, 2020·International Journal of Biological Macromolecules·Muralidharan VanuopadathSudarslal Sadasivan Nair
Dec 24, 2005·Toxicon : Official Journal of the International Society on Toxinology·Ponlapat RojnuckarinTanin Intragumtornchai