PMID: 9637365Jun 24, 1998Paper

Characterization of three fibrinogenolytic enzymes from Chinese green tree viper (Trimeresurus stejnegeri) venom

Toxicon : Official Journal of the International Society on Toxinology
R GaoW Y Wang

Abstract

From the venom of Chinese green tree viper (Trimeresurus stejnegeri), three distinct fibrinogenolytic enzymes: stejnefibrase-1, stejnefibrase-2 and stejnefibrase-3, were purified by gel filtration, ion-exchange chromatography and reverse-phase high-performance chromatography (HPLC). SDS-PAGE analysis of those three enzymes showed that they consisted of a single polypeptide chain with mol. wt of 50000, 31000 and 32000, respectively. Like TSV-PA (a specific plasminogen activator) and stejnobin (a fibrinogen-clotting enzyme) purified from the same venom, stejnefibrase-1, -2 and -3 were able to hydrolyze several chromogenic substrate. On the other hand, different from TSV-PA and stejnobin, stejnefibrase-1, -2 and -3 did not activate plasminogen and did not possess fibrinogen-clotting activity. The three purified enzymes directly degraded fibrinogen to small fragments and rendered it unclottable by thrombin. Stejnefibrase-2 degraded preferentially Bbeta-chain while stejnefibrase-1 and -3 cleaved concomitantly Aalpha and Bbeta-chains of fibrinogen. None of these proteases degraded the gamma-chain of fibrinogen. When correlated with the loss of clottability of fibrinogen, the most active enzyme was stejnefibrase-1. The activities of t...Continue Reading

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Citations

Apr 18, 2001·Toxicon : Official Journal of the International Society on Toxinology·Y JinY L Xiong
Aug 15, 2002·Toxicon : Official Journal of the International Society on Toxinology·D K JagadeeshaK Kemparaju
Jul 16, 2008·Toxicon : Official Journal of the International Society on Toxinology·Rong GaoPonnampalam Gopalakrishnakone
Mar 10, 2007·Toxicon : Official Journal of the International Society on Toxinology·Chuanchom Muanpasitporn, Ponlapat Rojnuckarin
Aug 30, 2008·Journal of Biochemical and Molecular Toxicology·Suphan SoogarunTusrin Moungkote
Jul 11, 2012·The Journal of Pharmacy and Pharmacology·Martin Flemmig, Matthias F Melzig
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Yang JinYu-Liang Xiong
Dec 7, 2005·Toxicon : Official Journal of the International Society on Toxinology·Shu-Rong ZhongYun Zhang
Dec 14, 2011·Toxicon : Official Journal of the International Society on Toxinology·José Antonio Valeriano-ZapanaSergio Marangoni
Aug 11, 2004·Toxicon : Official Journal of the International Society on Toxinology·Run-Qiang ChenYu-Liang Xiong
Oct 30, 2016·Bioengineered· Adivitiya, Yogender Pal Khasa
Dec 20, 2018·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Ângela Alice Amadeu MegaleWilmar Dias da Silva
Dec 24, 2005·Toxicon : Official Journal of the International Society on Toxinology·Ponlapat RojnuckarinTanin Intragumtornchai

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