Characterization of three new competence-regulated operons in Haemophilus influenzae

Journal of Bacteriology
Timothy M VanWagonerTerrence L Stull

Abstract

Haemophilus influenzae is one of a growing number of bacteria in which the natural ability to uptake exogenous DNA for potential genomic transformation has been recognized. To date, several operons involved in transformation in this organism have been described. These operons are characterized by a conserved 22-bp regulatory element upstream of the first gene and are induced coincident with transfer from rich to nutrient-depleted media. The previously identified operons comprised genes encoding proteins that include members of the type II secretion system and type IV pili, shown to be essential for transformation in other bacteria, and other proteins previously identified as required for transformation in H. influenzae. In the present study, three novel competence operons were identified by comparative genomics and transcriptional analysis. These operons have been further characterized by construction of null mutants and examination of the resulting transformation phenotypes. The putative protein encoded by the HI0366 gene was shown to be essential for DNA uptake, but not binding, and is homologous to a protein shown to be required for pilus biogenesis and twitching motility in Pseudomonas aeruginosa. An insertion in HI0939 abo...Continue Reading

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Citations

May 28, 2009·Applied Microbiology and Biotechnology·Naoto Yoshida, Misa Sato
Oct 28, 2006·Nucleic Acids Research·Andrew D S Cameron, Rosemary J Redfield
Jul 21, 2006·Journal of Bacteriology·Paul W WhitbyTerrence L Stull
Jun 9, 2009·Journal of Bacteriology·Sunita SinhaRosemary J Redfield
Oct 14, 2006·BMC Evolutionary Biology·Rosemary J RedfieldJohn He Nash
Apr 20, 2010·BMC Microbiology·Daniel J MortonTerrence L Stull
Nov 19, 2008·BMC Molecular Biology·Erin TracyRobert S Munson
Aug 26, 2009·BMC Research Notes·Daniel J MortonTerrence L Stull
Apr 26, 2012·PloS One·Sunita Sinha, Rosemary J Redfield
Oct 11, 2012·PloS One·Christina S VeggeHanne Ingmer
Feb 26, 2009·Evolution; International Journal of Organic Evolution·Heather Maughan, Rosemary J Redfield
Jan 16, 2010·Microbiology·Paul W WhitbyTerrence L Stull
Sep 15, 2014·Veterinary Microbiology·Nehal ShahThomas J Inzana

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