Characterization of three rice basic/leucine zipper factors, including two inhibitors of EmBP-1 DNA binding activity.

The Journal of Biological Chemistry
A Nantel, R S Quatrano

Abstract

The promoter of the wheat Em gene contains elements with a CACGTG core sequence (G-boxes), which are recognized by EmBP-1, a wheat basic/leucine zipper (bZIP) protein. G-boxes are required for Em expression in response to the phytohormone abscisic acid and for transactivation by the Viviparous-1 protein (VP1) using transient expression systems. In order to identify other factors that are part of the transcriptional complex that associates with G-boxes, we have screened a rice (Oryza sativa) cDNA library with biotinylated EmBP-1. We have isolated osZIP-1a, a homolog of EmBP-1 and other plant G-box-binding factors. We show that EmBP-1 and osZIP-1a will preferentially heterodimerize in vitro. Overexpression of osZIP-1a in rice protoplasts can enhance expression from the Em promoter only in the presence of abscisic acid. Two other clones have been identified by screening with EmBP-1: osZIP-2a and osZIP-2b. These osZIP-2 factors represent a novel class of bZIP proteins with an unusual DNA-binding domain that does not recognize G-boxes. The osZIP-2 factors can heterodimerize with EmBP-1 and prevent it from binding to the Em promoter. Interestingly, osZIP-1a does not heterodimerize with the osZIP-2 factors and its DNA binding activity...Continue Reading

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Citations

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Nov 28, 2001·European Journal of Biochemistry·Y SibérilP Gantet
Mar 6, 2009·Journal of Experimental Botany·Andrés P GageteM Isabel Rodrigo
Sep 12, 2002·Annual Review of Plant Biology·Jian-Kang Zhu
Jan 1, 2002·The Arabidopsis Book·Ruth R Finkelstein, Christopher D Rock
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·T HoboT Hattori
Sep 8, 2011·Plant Signaling & Behavior·Marjan M Tajrishi, Narendra Tuteja
Sep 1, 2014·Molecular Biotechnology·Ehsan PourabedZahra-Sadat Shobbar
Feb 14, 2002·Plant, Cell & Environment·S. Ramanjulu, D. Bartels
Nov 13, 2001·The Journal of Biological Chemistry·Srinivas S L GampalaChristopher D Rock
Jul 17, 2001·Physiologia Plantarum·Liming Xiong, Jian-Kang Zhu

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