Characterization of thymus atrophy in calves with subclinical BVD challenged with BHV-1

Veterinary Microbiology
F Romero-PalomoJ C Gómez-Villamandos

Abstract

Since the thymus is a target organ for the bovine viral diarrhea virus (BVDV), our experiment aimed to understand its relationship with the immunosuppressive effect by studying the consequences of a previous infection with BVDV on the thymus of calves challenged with bovine herpesvirus 1.1 (BHV-1). For this purpose, 12 animals were inoculated intranasally with non-cytopathic BVDV-1; 12 days later, 10 of them were coinfected intranasally with BHV-1. These animals were euthanized in batches of two at 0, 1, 2, 4, 7 or 14 dpi with BHV-1. Another 10 calves were inoculated solely with BHV-1 and euthanized in batches of two at 1, 2, 4, 7 or 14 dpi with BHV-1; two uninoculated calves were used as negative controls. Thymus samples from these animals were processed for viral detection and histopathological, immunohistochemical, and ultrastructural studies focused on BVDV/BHV-1 antigens, cortex:medulla ratio, apoptosis (TUNEL and caspase-3), collagen deposition, and factor VIII endothelial detection. Our study revealed the immunohistochemical presence of BVDV antigen in all animals in the BVDV-infected group, unlike BHV-1 detection, which was observed in animals in both infection groups only by molecular techniques. BVDV-preinfected anima...Continue Reading

References

Jan 1, 1996·Cell Biology International·G S Montes
Nov 16, 2001·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M AlegreN Fondevila
Nov 22, 2002·Journal of Comparative Pathology·P J Sánchez-CordónJ C Gómez-Villamos
May 9, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Elisabeth M Liebler-TenorioJohn D Neill
May 29, 2003·Biologicals : Journal of the International Association of Biological Standardization·E M Liebler-TenorioJ D Neill
Jul 26, 2003·Burns : Journal of the International Society for Burn Injuries·Paul P M van ZuijlenEsther Middelkoop
Jan 30, 2004·Journal of Virology·Karin MaurerTill Rümenapf
Sep 28, 2004·The Journal of General Virology·Ana R ResendesMariano Domingo
Jul 19, 2006·PLoS Pathogens·Wilson Savino
Aug 14, 2012·Biologicals : Journal of the International Association of Biological Standardization·John D Neill
Jan 8, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Robert B MoellerPatricia C Blanchard
Mar 27, 2013·Veterinary Pathology·F Romero-PalomoJ C Gómez-Villamandos

❮ Previous
Next ❯

Citations

Aug 26, 2015·Transboundary and Emerging Diseases·F Romero-PalomoJ C Gómez-Villamandos
Mar 24, 2017·Archivum Immunologiae Et Therapiae Experimentalis·Abdur Rahman Ansari, Huazhen Liu
Jun 12, 2021·Frontiers in Immunology·Mingli LuoXi Sun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis