Characterization of thyroglobulin epitopes in Sardinian adults and juveniles with Hashimoto's thyroiditis: evidence against a major effect of age and genetic background on B-cell epitopes

Clinical Endocrinology
Francesco LatrofaStefano Mariotti

Abstract

Using recombinant human monoclonal thyroglobulin antibodies expressed as Fab molecules (TgAb-Fab), we have recently confirmed the restriction of Tg epitopes in Hashimoto's thyroiditis (HT). To investigate Tg epitopes of serum TgAb in HT adults and HT juveniles from a geographically isolated area (Sardinia). Serum TgAb of 39 Sardinian HT adults, 53 Sardinian HT juveniles and 45 non-Sardinian HT adults were evaluated. The binding of serum TgAb to Tg in ELISA was inhibited by four recombinant human TgAb-Fab, identifying Tg epitopic regions A-D. The percentage of Tg binding inhibition was calculated comparing the binding of serum TgAb in presence of each TgAb-Fab with that in its absence. In the whole cohort of 137 patients, A region TgAb-Fab induced the highest levels of inhibition (55.3 +/- 17.8%) (mean +/- SD). Lower levels of inhibition were induced by TgAb-Fab of regions B (27.8 +/- 25.8%), C (26.8 +/- 24.6%) and D (17.5 +/- 18.4%). In Sardinian HT adults inhibition by TgAb-Fab of regions A, B and C were comparable to Sardinian HT juveniles; the marginal D region TgAb-Fab induced a slightly higher inhibition (22.1 vs. 13.8%; P = 0.034) in the former than in the latter group. In Sardinian and non-Sardinian HT adults inhibitions...Continue Reading

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Citations

Oct 15, 2014·International Journal of Clinical Oncology·Hee Yong KwakJa Seong Bae
Jan 10, 2013·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Yu LunJian Zhang
Sep 28, 2012·Journal of Endocrinological Investigation·F LatrofaP Vitti
Feb 15, 2018·Thyroid : Official Journal of the American Thyroid Association·Francesco LatrofaPaolo Vitti
Mar 21, 2013·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Roberto NegroSimonetta Piana
Jan 31, 2020·The Journal of Clinical Endocrinology and Metabolism·Debora RicciFrancesco Latrofa
Jan 27, 2012·The Journal of Clinical Endocrinology and Metabolism·Emilio Fiore, Paolo Vitti
Mar 30, 2016·Thyroid : Official Journal of the American Thyroid Association·Francesco LatrofaPaolo Vitti

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