Characterization of tight junction disruption and immune response modulation in a miniaturized Caco-2/U937 coculture-based in vitro model of the human intestinal barrier

Biomedical Microdevices
Qasem Ramadan, Lin Jing

Abstract

A microfluidic-based dynamic in vitro model of the human intestinal barrier has been constructed and characterized. The intestinal epithelial monolayer was mimicked by culturing caco-2 cells on a porous membrane in a double-layered microfluidic chip and interfaced with a co-culture of U937 as a model of immune responsive cells. The physiological flow was also mimicked by a continuous perfusion of culture media from the apical and basolateral side of the porous membrane. This dynamic "in vivo-like" environment maintains a continuous supply of cell nutrient and waste removal and create mechanical shear stress within the physiological ranges which promotes uniform cell growth and tight junction formation. The monolayer permeability to soluble ion changes after treating with LPS, and TNF α as indicated by the reduction of the TEER value. In addition, the immune competent caco-2/U937-based model allowed the investigating the role of the epithelial layer as a protection barrier to biological hazards as indicated by the suppressing of the pro-inflammatory cytokine expression.

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Citations

Jul 24, 2020·Biomicrofluidics·Qasem Ramadan, Mohammed Zourob
Apr 30, 2017·Biomedical Microdevices·Kyu-Young ShimJong Hwan Sung
Oct 4, 2017·Scientific Reports·Cait M CostelloJohn C March
Oct 3, 2018·Critical Reviews in Food Science and Nutrition·Maria Del Carmen Ponce de León-RodríguezCaroline Laurent-Babot
Oct 9, 2019·Advanced Healthcare Materials·Chiara Anna Maria FoisFariba Dehghani
Jan 23, 2021·Critical Reviews in Food Science and Nutrition·Duo LiangMingqian Tan
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Chrysanthi-Maria MoysidouRóisín Meabh Owens
Dec 8, 2020·Journal of Tissue Engineering·Yunqing XiangShuqiang Huang
Aug 15, 2021·Advanced Drug Delivery Reviews·Connor O'FarrellHannah Batchelor

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