Characterization of tomato endo-beta-1,4-glucanase Cel1 protein in fruit during ripening and after fungal infection

Planta
María Dolores RealCarmen González-Bosch

Abstract

The tomato (Lycopersicon esculentum Mill.) endo-beta-1,4-glucanase (EGase) Cel1 protein was characterized in fruit using specific antibodies. Two polypeptides ranging between 51 and 52 kDa were detected in the pericarp, and polypeptides ranging between 49 and 51 kDa were detected in locules. The polypeptides recognized by Cel1 antiserum in fruit are within the size range predicted for Cel1 protein and could be derived from heterogeneous glycosylation. Cel1 protein accumulation was examined throughout fruit ripening. Cel1 protein appears in the pericarp at the stage in which many ripening-related changes start, and remains present throughout fruit ripening. In locules, Cel1 protein is already present at the onset of fruit ripening and remains constant during fruit ripening. This pattern of expression supports a possible role for this EGase in the softening of pericarp tissue and in the liquefaction of locules that takes place during ripening. The accumulation of Cel1 protein was also analyzed after fungal infection. Cel1 protein and mRNA levels are down-regulated in pericarp after Botrytis cinerea infection but are not affected in locular tissue. The same behavior was observed when fruits were infected with Penicillium expansum,...Continue Reading

References

Jan 1, 1990·Annual Review of Biochemistry·D J Bowles
Jul 1, 1995·Plant Molecular Biology·S B Milligan, C S Gasser
Jul 1, 1996·Plant Physiology·E del Campillo, A B Bennett
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·D A BrummellA B Bennett
Feb 20, 1999·The Biochemical Journal·W R McLauchlanJ Maat
Sep 7, 2002·The Plant Cell·John P VogelShauna C Somerville
Apr 1, 1994·Plant Physiology·J. D. Walton
May 3, 2003·Biochemical and Biophysical Research Communications·Patricia Company, Carmen González-Bosch
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D DellapennaA B Bennett
Oct 1, 1987·Plant Physiology·D DellapennaA B Bennett

❮ Previous
Next ❯

Citations

Oct 6, 2007·The Plant Journal : for Cell and Molecular Biology·Víctor FlorsCarmen González-Bosch
Oct 15, 2013·Physiologia Plantarum·Tirupaati Swaroopa Rani, Appa Rao Podile
Apr 9, 2020·Annals of Botany·Laila ToumGustavo E Gudesblat
Sep 27, 2019·International Journal of Molecular Sciences·Svante ResjöErik Andreasson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.