Characterization of tomato protein kinases embedding guanylate cyclase catalytic center motif.

Scientific Reports
Hafizur RahmanXin-Zhong Cai

Abstract

Guanylate cyclases (GCs) are enzymes that catalyze the reaction to produce cyclic GMP (cGMP), a key signaling molecule in eukaryotes. Nevertheless, systemic identification and functional analysis of GCs in crop plant species have not yet been conducted. In this study, we systematically identified GC genes in the economically important crop tomato (Solanum lycopersicum L.) and analyzed function of two putative tomato GC genes in disease resistance. Ninety-nine candidate GCs containing GC catalytic center (GC-CC) motif were identified in tomato genome. Intriguingly, all of them were putative protein kinases embedding a GC-CC motif within the protein kinase domain, which was thus tentatively named as GC-kinases here. Two homologs of Arabidopsis PEPRs, SlGC17 and SlGC18 exhibited in vitro GC activity. Co-silencing of SlGC17 and SlGC18 genes significantly reduced resistance to tobacco rattle virus, fungus Sclerotinia sclerotiorum, and bacterium Pseudomonas syringae pv. tomato (Pst) DC3000. Moreover, co-silencing of these two genes attenuated PAMP and DAMP-triggered immunity as shown by obvious decrease of flg22, chitin and AtPep1-elicited Ca2+ and H2O2 burst in SlGC-silenced plants. Additionally, silencing of these genes altered the...Continue Reading

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Citations

Feb 13, 2021·International Journal of Molecular Sciences·Ilona Turek, Helen Irving
Jan 21, 2021·International Journal of Molecular Sciences·Edwin Jarratt-BarnhamJulia M Davies
Mar 6, 2021·Frontiers in Plant Science·Brygida Świeżawska-BonieckaKrzysztof Jaworski
Jul 3, 2021·International Journal of Molecular Sciences·Maria DuszynAdriana Szmidt-Jaworska

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Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

BLAST
BLASTp
SPSS
FlexControl
GCPred
GeneDoc
FlexAnalysis
MEGA
clustalW2
phi

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