Characterization of torovirus from human fecal specimens

Virology
L DuckmantonM Petric

Abstract

The toroviruses, Berne virus (BEV) and Breda virus (BRV), are recognized pathogens of horses and cattle, respectively. Torovirus-like particles (TVLPs) that are immunologically related to BRV have been reported as etiological agents of gastroenteritis in humans. Of the toroviruses, only BEV has been shown to replicate in cell culture. Hence, these agents can be routinely detected only by electron microscopy (EM), although serological testing has been used as well. Our studies have provided supporting evidence that the TVLPs detected in the stool specimens of pediatric patients with gastroenteritis are human toroviruses. By EM, these particles are morphologically similar to BEV and BRV. Thin-section electron microscopy revealed that TVLPs contain toroidal-shaped nucleocapsids. Viruses purified from human fecal specimens agglutinate rabbit erythrocytes. BRV antiserum as well as convalescent sera from patients with gastroenteritis whose stools contain TVLPs were shown to contain antibodies that react with purified TVLPs as demonstrated by hemagglutination inhibition, immunoelectron microscopy, and immunoblotting. RNA extracted from partially purified TVLP preparations is amplifiable by RT-PCR using primers bracketing a 219-base re...Continue Reading

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Citations

Jun 30, 2005·Animal Health Research Reviews·Armando E Hoet, Linda J Saif
Mar 16, 2007·Journal of Clinical Microbiology·Sanela SvrakaMarion Koopmans
Dec 12, 2012·BMC Research Notes·Julio Alonso-PadillaDolores Rodríguez
Jan 15, 2014·Archives of Virology·Irem GülaçtıIbrahim Sözdutmaz
Sep 9, 2004·Current Opinion in Infectious Diseases·Benjamin Clark, Mike McKendrick
Feb 4, 2010·Archives of Virology·Dong-Jun ShinKyoung-Oh Cho
Dec 29, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mika ItoMakoto Nagai
Aug 28, 2007·Veterinary Microbiology·Su-Jin ParkKyoung-Oh Cho
Jul 15, 2003·Journal of Medical Virology·Djin-Ye OhEckart Schreier
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Dec 20, 2014·Genome Announcements·Srivishnupriya AnbalaganKaren Schwartz
May 9, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Armando E HoetLinda J Saif
Mar 29, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Armando E HoetLinda J Saif
Apr 4, 2021·Viruses·Makoto Ujike, Fumihiro Taguchi
Apr 29, 2011·European Journal of Medicinal Chemistry·Mathew StanleyHansjörg Streicher
Nov 25, 2021·Journal of Virology·Makoto UjikeWataru Kamitani

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