Characterization of Toxoplasma gondii isolates in free-range chickens from Portugal

The Journal of Parasitology
J P DubeyP Thulliez

Abstract

The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii in 225 free-range chickens (Gallus domesticus) from Portugal was determined. Antibodies to T. gondii were assayed by the modified agglutination test (MAT) and found in 61 chickens with titers of 1:5 in 8, 1:10 in 6, 1:20 in 3, 1:40 in 23, 1:80 in 5, 1:160 in 4, 1:320 in 8, and 1:640 or higher in 4. Hearts, leg muscles, and brains of 15 seropositive (MAT 1:10 or higher) chickens were bioassayed individually in mice. Tissue from 38 chickens with titers of 1:5 or less were pooled and fed to a T. gondii-free cat. Feces of the cat were examined for oocysts, but none was found. Toxoplasma gondii was isolated from 16 of 19 chickens with MAT titers of 1:10 or higher. Genotyping of 12 of these 16 isolates with polymorphisms at the SAG2 locus indicated that 4 were type III, and 8 were type II. None of the isolates was lethal for mice. Phenotypically, T. gondii isolates from chickens from Portugal were different from those of T. gondii isolates from chickens from Brazil.

References

Jul 1, 1987·Equine Veterinary Journal·J P Dubey, G Desmonts
Dec 1, 1995·The Journal of Infectious Diseases·D K Howe, L D Sibley
Jul 28, 2001·The Journal of Infectious Diseases·M E GriggT P Margolis
Jun 11, 2002·Comparative Immunology, Microbiology and Infectious Diseases·G JungersenP Lind
Jan 23, 2003·The Journal of Parasitology·Nuno CanadaJ P Dubey
Jun 18, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Tovi LehmannJ P Dubey
Nov 25, 2003·The Journal of Parasitology·J P DubeyT Y Morishita
Nov 25, 2003·The Journal of Parasitology·J P DubeyT Lehmann
Jan 20, 2004·Veterinary Parasitology·C SreekumarJ P Dubey
May 26, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Tovi LehmannJ P Dubey
May 29, 2004·The Journal of Parasitology·J P DubeyT Lehmann
Sep 24, 2004·International Journal for Parasitology·D AjzenbergM L Dardé
Dec 28, 2004·Veterinary Parasitology·Aristeu Vieira da SilvaHelio Langoni

❮ Previous
Next ❯

Citations

Jan 20, 2010·Veterinary Parasitology·Susana SousaMarie-Laure Dardé
Aug 10, 2011·Veterinary Parasitology·Jered M WendteMichael E Grigg
Jan 25, 2011·Veterinary Parasitology·Rodrigo Martins SoaresHilda Fátima de Jesus Pena
Apr 5, 2014·Comparative Immunology, Microbiology and Infectious Diseases·Marija MarkovićOlgica Djurković-Djaković
Nov 7, 2014·Journal of Wildlife Diseases·Rafael Calero-BernalIsabel Fuentes
Dec 12, 2013·Avian Pathology : Journal of the W.V.P.A·Shuai WangXiangRui Li
Apr 6, 2017·Parasites & Vectors·Ivana KlunOlgica Djurković-Djaković
May 2, 2007·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Cheryl RosaJohn Blake
Sep 6, 2017·Asian Pacific Journal of Tropical Medicine·Azar ShokriAhmad Daryani
Jun 27, 2019·Acta Veterinaria Hungarica·Ljiljana KurucaOlgica Djurković-Djaković

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.