PMID: 9190811Jun 1, 1997Paper

Characterization of transcription organization and analysis of unique expression patterns of an alkyl hydroperoxide reductase C gene (ahpC) and the peroxide regulator operon ahpF-oxyR-orfX from Xanthomonas campestris pv. phaseoli

Journal of Bacteriology
Skorn MongkolsukS Atichartpongkun

Abstract

We have analyzed the transcription organization of ahpC, ahpF, oxyR, and orfX from Xanthomonas campestris pv. phaseoli. ahpC was transcribed as a monocistronic 0.6-kb mRNA, while ahpF-oxyR-orfX were transcribed as a polycistronic approximately 3.0-kb-long mRNA. The novel transcription organization of these genes has not observed in other bacteria. Western analysis showed that oxidants (peroxides and superoxide anions), a thiol reagent (N-ethylmaleimide), and CdCl2 caused large increases in the steady-state level of AhpC. Growth at alkaline pH also moderately induced AhpC accumulation. Thermal and osmotic stresses did not alter the levels of AhpC. Northern blotting results confirmed that oxidant- and CdCl2-induced AhpC accumulation was due to increased levels of ahpC transcripts. Analysis of oxyR expression revealed a unique pattern. Unlike other bacterial systems, peroxides and a superoxide generator induced accumulation of OxyR. Northern blotting results confirmed that these oxidants induced expression of oxyR operon. This novel regulatory pattern could be generally important. The transcription organization and patterns of chemicals and stress induction of ahpC and oxyR differed from those of other bacteria and are likely to b...Continue Reading

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Citations

Jul 9, 2002·Molecular Microbiology·Skorn Mongkolsuk, John D Helmann
Apr 17, 2009·FEMS Microbiology Reviews·Thomas Girard ChasteenClaudio Christian Vásquez
Sep 30, 2000·European Journal of Biochemistry·L B PooleM Li Calzi
Aug 16, 2006·FEMS Microbiology Letters·Karnjana HrimpengSkorn Mongkolsuk
May 8, 2002·Molecular Microbiology·Skorn MongkolsukSupa Utamapongchai
Feb 7, 2001·Microbiology·P VattanaviboonS Mongkolsuk
Jun 13, 2000·Journal of Bacteriology·S MongkolsukS Loprasert
Dec 17, 2004·Journal of Bacteriology·Alexandre JametDidier Hérouart
Oct 10, 1998·Journal of Bacteriology·A H van VlietJ M Ketley
May 14, 1999·Current Opinion in Microbiology·G Storz, J A Imlay
Oct 3, 2002·Biochemical and Biophysical Research Communications·Paiboon VatanaviboonSkorn Mongkolsuk
Jul 18, 2012·Journal of Proteome Research·Anna ManaraAntonella Furini

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