Characterization of transgenic pigs that express human decay accelerating factor and cell membrane-tethered human tissue factor pathway inhibitor

Reproduction in Domestic Animals = Zuchthygiene
H J LeeH M Rho

Abstract

The expression of human complement regulatory proteins (hCRP; hDAF, hCD59, and hMCP) in pig tissues has been suggested as one of strategies to overcome the hyperacute rejection (HAR) in pig-to-human transplantation. Expression of human tissue factor pathway inhibitor (hTFPI) in porcine endothelial cells has been suggested as a remedy to overcome microvascular thrombosis. To investigate the effects of these combined transgenes, we established transformed pig cells expressing human decay accelerating factor (hDAF) under the control of enhancer promoter (5'LTR-PCMVIE), and the fusion protein (hTFPI/hCD4) consisting of the functional domains (K1 and K2) of hTFPI and membrane-tethering domains (D3 and D4) of hCD4 under the control of PCMVIE. Transgenic pigs were generated with the transformed porcine cells through somatic cell nuclear transfer (SCNT) technology. Analysis of quantitative PCR and real-time quantitative RT-PCR showed that four copies of hDAF were integrated and 391 copies of hDAF mRNA expressed in the cells of the transgenic pig. The enhancing activity of 5'LTR was approximately 2 fold compared to CMVIE promoter only. The cell viability test showed that more than 80% of ear cells were viable in the presence of 50% huma...Continue Reading

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Citations

Mar 29, 2012·Journal of Inherited Metabolic Disease·Yonglun LuoCharlotte Brandt Sørensen
Mar 19, 2011·Current Opinion in Organ Transplantation·Peter J CowanAnthony J F d'Apice
Feb 9, 2012·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Xiurong YangHesheng Jiang
Oct 4, 2013·Kidney International·Peter J CowanAnthony J F d'Apice
Jun 12, 2012·Critical Reviews in Biochemistry and Molecular Biology·Jennifer K Dowling, Luke A J O'Neill
Aug 9, 2017·Balsaeng'gwa saengsig·Seung-Chan LeeSeongsoo Hwang
Jan 8, 2021·International Journal of Immunogenetics·Marco Carvalho-OliveiraConstanca Figueiredo
Apr 11, 2020·Advanced Drug Delivery Reviews·Dong NiuGeorge M Church

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