PMID: 8973557Dec 1, 1996Paper

Characterization of transgenic potato (Solanum tuberosum) tubers with increased ADPglucose pyrophosphorylase

The Biochemical Journal
Lee SweetloveT ap Rees

Abstract

The aim of the work described in this paper was to characterize the tubers of potato (Solanum tuberosum var. Prairie) plants that had been transformed with the Escherichia coli ADPglucose pyrophosphorylase (EC 2.7.7.27) gene, glgC-16, under the control of a patatin promoter. Over 30 lines of transformed plants with increased ADPglucose pyrophosphorylase activity were obtained. The tubers of six of these lines were compared with those of control plants expressing the gene for beta-glucuronidase. The average increase in pyrophosphorylase activity was 200%, and the highest was 400%. Western immunoblotting of tuber extracts showed that the amounts of glgC-16 protein were linearly related to the extractable activity of the ADPglucose pyrophosphorylase. Cell fractionation studies showed that the increased activity of the pyrophosphorylase in the glgC-16 tubers had a similar intracellular location, the amyloplast fraction, to that found in the control tubers. No pleiotropic changes in the maximum catalytic activities of the following enzymes could be detected in the glgC-16 tubers: sucrose synthase, fructokinase, UDPglucose pyrophosphorylase, phosphofructokinase, soluble starch synthase, starch branching enzyme, phosphoglucomutase and...Continue Reading

Citations

Jun 24, 2009·Plant Biotechnology Journal·Jean Louis PrioulKeith J Edwards
Apr 2, 2013·Annual Review of Chemical and Biomolecular Engineering·Jong Moon YoonJacqueline V Shanks
Sep 20, 2008·Plant & Cell Physiology·Francisco José MuñozJavier Pozueta-Romero
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·U RoessnerL Willmitzer
Dec 21, 2006·Plant Biotechnology Journal·Uzoma IhemereRichard Sayre
Feb 8, 2005·Plant & Cell Physiology·Charles J BaxterLee J Sweetlove
Jun 1, 2008·Metabolomics : Official Journal of the Metabolomic Society·Eva M FarreLothar Willmitzer
Aug 31, 2012·Plant Biotechnology Journal·Claudia JonikFrank Ludewig
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Edurne Baroja-FernándezJavier Pozueta-Romero
Sep 1, 1998·The Plant Journal : for Cell and Molecular Biology·Lee J SweetloveSteven A Hill
Nov 19, 2019·Frontiers in Plant Science·Rupesh TayadeJeong-Dong Lee
Sep 17, 2009·Plant Physiology·Mark G PoolmanDavid A Fell

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