Characterization of transport systems for the transfer of 3,4-L-dihydroxyphenylalanine into slices of rat cerebral cortex.

Biochimica Et Biophysica Acta
F J Garcia-Sancho, B Herreros

Abstract

1. Slices of rat cerebral cortex incubated aerobically at 37 degrees C in Krebs-Ringer-bicarbonate solution accumulated 3,4-L-dihydroxyphenylalanine (L-DOPA) against its concentration gradient. With 1 mM L-DOPA in the medium, tissue-water/medium concentration ratios of about 6 : 1 are reached, which are modified by the presence of other amino acids in the medium. 2. Kinetic analysis suggested that L-DOPA influx into brain cells occurred by at least two saturable processes, which show apparent Km values in the range of 10(-3) M and 10(-5) M, respectively. 3. Prior incubation of the slices in Na+-free (choline-containing) medium at 37 degrees C depressed their subsequent uptake of L-DOPA in normal Na+-containing medium; this inhibition did not appear when the preincubation was carried out at 0-4 degrees C. Besides this effect of preincubation, most of L-DOPA influx into brain slices was independent of the actual concentration of Na+ in the medium; the two saturable processes described in this article behaved similarly in this respect. 4. Most of L-DOPA uptake by the high-Km process is mediated by an agency that resembles the Na+-independent L system described in Ehrlich cells (Oxender, D. L. and Christensen, H. N. (1963) J. Biol....Continue Reading

References

Dec 1, 1972·Journal of Neurochemistry·M Kiely, T L Sourkes
Sep 1, 1972·Neuropharmacology·R J Baldessarini, M Karobath
Sep 1, 1972·Journal of Neurochemistry·Y P LiuM H Van Woert
Dec 1, 1971·The American Journal of Physiology·W H Oldendorf
May 1, 1971·European Journal of Pharmacology·M KarobathM O Huttunen
Sep 1, 1970·Journal of Neurochemistry·D G Grahame-Smith, A G Parfitt
Sep 3, 1968·Biochimica Et Biophysica Acta·H N Christensen
Nov 5, 1968·Biochimica Et Biophysica Acta·R K Margolis, A Lajtha
Feb 1, 1964·Journal of Neurochemistry·S LAHIRI, A LAJTHA

❮ Previous
Next ❯

Citations

Mar 1, 1987·Journal of the Autonomic Nervous System·E GonzalezC Gonzalez
Sep 4, 1979·Biochimica Et Biophysica Acta·J Garcia-SanchoB Herreros
Oct 12, 2001·Journal of Neurochemistry·M L MundorfR M Wightman
Sep 1, 2005·Neuroscience and Behavioral Physiology·V I Mel'nikovaM V Ugryumov
Feb 15, 1996·Journal of Neuroscience Research·M J Tsai, E H Lee
Jan 22, 2005·The Journal of Biological Chemistry·Susanna BodoyJoan Bertran
Jul 20, 1981·Biochimica Et Biophysica Acta·A Floud, S Fahn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.