PMID: 9429158Jan 16, 1998Paper

Characterization of troponin-C interactions in skinned barnacle muscle: comparison with troponin-C from rabbit striated muscle

Journal of Muscle Research and Cell Motility
A M GordonD A Martyn

Abstract

Previously it was shown that when troponin-C (TnC) is extracted from barnacle myofibrillar bundles they lose their Ca2+ sensitivity, which can be restored by adding back barnacle TnC (either isoform, BTnC1 or BTnC2). Thus barnacle muscle shows thin filament regulation, as does rabbit psoas skeletal muscle. In this paper we compare the interactions of barnacle and rabbit fast muscle TnC in their respective muscles. We demonstrate that muscle fibres from the giant barnacle, Balanus nubilus, contain about 186 microM kg-1 muscle tissue of BTnC1 plus BTnC2 compared to about 91 microM kg-1 of TnC in rabbit psoas muscle fibres. Extraction of BTnC is achieved using similar low ionic strength, low divalent ion Ca(2+)-low Mg2+ conditions which are required for TnC extraction in rabbit psoas skinned muscle fibres; extraction was prevented by 1 mM Mg2+. Full reconstitution of Ca(2+)-sensitivity was achieved by adding back BTnC (1 + 2, or 2). Reconstitution of barnacle muscle with rabbit fast skeletal TnC (RTnC) was more complex, with partial recovery of Ca(2+)-sensitivity with reconstitution in the presence of 3 mM Mg2+ and more fully with reconstitution in the presence of activating Ca2+ (pCa 4.0). This suggests that the barnacle TnC-TnI ...Continue Reading

Citations

Jul 2, 2004·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Annette KoendersDonald L Mykles
Nov 19, 2011·American Journal of Physiology. Cell Physiology·Anabelle S Cornachione, Dilson E Rassier
Oct 12, 2007·American Journal of Physiology. Cell Physiology·V JoumaaW Herzog
Apr 17, 2015·PloS One·David AltmanDilson E Rassier
Sep 26, 2015·American Journal of Physiology. Cell Physiology·Anabelle S CornachioneDilson E Rassier
Jun 16, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Erica ChaoDonald L Mykles

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