PMID: 9651938Jul 4, 1998Paper

Characterization of trypanosome infections by polymerase chain reaction (PCR) amplification in wild tsetse flies in Cameroon

Parasitology
I MorlaisG Cuny

Abstract

The polymerase chain reaction (PCR) method was used to characterize trypanosome infections in tsetse flies from 3 sleeping sickness foci in Cameroon. The predominant tsetse species found was Glossina palpalis palpalis. An average infection rate of 12.1% was revealed by microscopical examination of 888 non-teneral tsets flies. PCR amplification analyses for trypanosome identification were carried out on 467 flies, with primer sets specific for Trypanosoma (Trypanozoon) brucei s.1., T. (Duttonella) vivax, T. (Nannomonas) simiae and forest type T. (Nannomonas) congolense. Of 467 flies 93 were positive by microscopical analysis while PCR succeeded in identifying 89 positive flies. Of the PCR-positive flies 34 (38.2%) were negative by microscopical examination. PCR amplification, when compared to the parasitological technique, gave a higher estimate of infection rate of trypanosomes in natural tsetse populations. The PCR technique did, however, fail to identify 40.9% (38/93) of the parasitologically positive flies. The reasons for this failure are discussed. The overall prevalence of mixed infections, assessed by PCR, was 37.1%; the majority (72.7%) involved T. brucei and forest type T. congolense.

Citations

Sep 8, 2010·Bulletin de la Société de pathologie exotique·L Kohagne TonguéF J Louis
Sep 11, 1999·Parasitology Today·S C Welburn, I Maudlin
Apr 12, 2003·Trends in Parasitology·Elliot S Krafsur
Nov 29, 2011·The Pan African Medical Journal·Tongué Lisette KohagneRémy Mimpfoundi
Apr 14, 2016·Parasites & Vectors·Pascal GrébautGustave Simo
Apr 30, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Gustave SimoTazoacha Asonganyi
May 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Emre AksoySerap Aksoy
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