Abstract
The polymerase chain reaction (PCR) method was used to characterize trypanosome infections in tsetse flies from 3 sleeping sickness foci in Cameroon. The predominant tsetse species found was Glossina palpalis palpalis. An average infection rate of 12.1% was revealed by microscopical examination of 888 non-teneral tsets flies. PCR amplification analyses for trypanosome identification were carried out on 467 flies, with primer sets specific for Trypanosoma (Trypanozoon) brucei s.1., T. (Duttonella) vivax, T. (Nannomonas) simiae and forest type T. (Nannomonas) congolense. Of 467 flies 93 were positive by microscopical analysis while PCR succeeded in identifying 89 positive flies. Of the PCR-positive flies 34 (38.2%) were negative by microscopical examination. PCR amplification, when compared to the parasitological technique, gave a higher estimate of infection rate of trypanosomes in natural tsetse populations. The PCR technique did, however, fail to identify 40.9% (38/93) of the parasitologically positive flies. The reasons for this failure are discussed. The overall prevalence of mixed infections, assessed by PCR, was 37.1%; the majority (72.7%) involved T. brucei and forest type T. congolense.
Citations
Sep 8, 2010·Bulletin de la Société de pathologie exotique·L Kohagne TonguéF J Louis
Jan 30, 2004·Veterinary Parasitology·P Van den BosscheD Berkvens
Sep 11, 1999·Parasitology Today·S C Welburn, I Maudlin
Apr 12, 2003·Trends in Parasitology·Elliot S Krafsur
Jun 10, 2010·PLoS Pathogens·Jan Van Den AbbeeleMarc Coosemans
Nov 29, 2011·The Pan African Medical Journal·Tongué Lisette KohagneRémy Mimpfoundi
Apr 3, 2014·Parasites & Vectors·Gustave SimoAsonganyi Tazoacha
Sep 8, 2004·Acta Tropica·F NjiokouS Herder
Mar 29, 2006·Veterinary Parasitology·G SimoS Herder
Nov 9, 2001·Acta Tropica·I MorlaisG Cuny
Apr 14, 2016·Parasites & Vectors·Pascal GrébautGustave Simo
Apr 30, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Gustave SimoTazoacha Asonganyi
Feb 16, 2002·Experimental Parasitology·E C AgboM H Roos
Mar 8, 2011·Experimental Parasitology·Gustave SimoTazoacha Asonganyi
Feb 10, 2006·Journal of Medical Entomology·C KubiP Van den Bossche
May 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Emre AksoySerap Aksoy
Nov 18, 2005·Molecular and Biochemical Parasitology·Chris Proudfoot, Richard McCulloch
Dec 21, 2017·Parasite : Journal De La Société Française De Parasitologie·Pythagore Soubgwi FogueGustave Simo
Aug 18, 2018·Parasite : Journal De La Société Française De Parasitologie·Sartrien Kanté TagueuGustave Simo
Jan 6, 2005·Vector Borne and Zoonotic Diseases·P GrébautC Laveissière
Jul 10, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Herakles A GarciaMarta M G Teixeira