PMID: 7545812Jun 1, 1995Paper

Characterization of two distinct Cl- conductances in fused human respiratory epithelial cells. II. Relation to cystic fibrosis gene product

Pflügers Archiv : European journal of physiology
U H Schröder, E Frömter

Abstract

The present microelectrode experiments on fused respiratory epithelial cells of cystic fibrosis (CF) origin and non-CF origin aim at characterizing the molecular basis of the Cl- conductances regulated by cyclic adenosine monophosphate (cAMP) or respectively Ca2+, as described in the preceding publication. Cell membrane potential (Vm) and resistance (Rm) were recorded as well as their response to substitution of 90% of bath Cl- by isethionate (delta Vm,ISE), by I- (delta Vm,I), or by other halide anions. Fused CF cells had significantly (P < 0.05) higher control Vm values (-18.0 +/- 9.4 mV, +/- SD, n = 68) than fused non-CF cells (-12.5 +/- 6.6 mV, n = 69) and responded to the Ca2+ ionophore A23187 with an increase in the Vm response to Cl- substitution, but did not respond to forskolin. This indicates that CF cells express only the Ca(2+)-stimulated Cl- conductance. Injection of the antibody M3A7 against a fusion protein containing amino acids 1195 to 1480 of the CF gene product into young, forskolin-stimulated or old non-CF cells decreased delta Vm,ISE and delta Vm,I within 15 min to values observed in CF cells. This indicates inhibition of the cAMP-stimulated Cl- conductance and supports the molecular identity of this conduc...Continue Reading

References

Aug 1, 1992·Nature Genetics·N KartnerJ R Riordan
Apr 24, 1992·Science·N A BradburyK L Kirk
Jun 1, 1991·Pflügers Archiv : European journal of physiology·K KunzelmannR Greger
Jun 1, 1991·Pflügers Archiv : European journal of physiology·W KrickE Frömter
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R A SchoumacherT A Rado
Mar 1, 1991·Pflügers Archiv : European journal of physiology·L J GaliettaG Romeo
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M P Anderson, M J Welsh
Jan 1, 1991·Advances in Experimental Medicine and Biology·J DisserE Frömter
Jan 1, 1991·Molecular Aspects of Medicine·M A McPherson, R L Dormer
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W H Cliff, R A Frizzell
Nov 1, 1987·Pflügers Archiv : European journal of physiology·J P HayslettR Greger
Apr 1, 1988·The American Journal of Physiology·D R HalmR A Frizzell
Aug 1, 1988·The Journal of General Physiology·R Horn, A Marty
Nov 1, 1989·Pflügers Archiv : European journal of physiology·K KunzelmannR Greger
Oct 1, 1989·The Journal of Membrane Biology·U KerstingH Oberleithner
Jul 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H M BerschneiderD W Powell
May 1, 1988·The American Journal of Physiology·J L GoldsteinM C Rao
Jan 1, 1969·Annual Review of Physiology·J M Diamond, E M Wright
Jan 1, 1984·Annual Review of Physiology·B Sakmann, E Neher
May 1, 1983·The Journal of Clinical Investigation·M KnowlesR Boucher
Nov 4, 1993·Nature·J A TabcharaniJ W Hanrahan
Oct 1, 1993·The Journal of General Physiology·C KorbmacherE L Boulpaep
Oct 1, 1959·The Journal of Physiology·A L HODGKIN, P HOROWICZ

❮ Previous
Next ❯

Citations

Oct 6, 1997·The Journal of General Physiology·J A TabcharaniJ W Hanrahan
Jul 20, 2004·Pflügers Archiv : European journal of physiology·Gabrielle Planelles

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.