PMID: 8946011Nov 13, 1996Paper

Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils

Journal of Immunological Methods
L KjeldsenNiels Borregaard

Abstract

NGAL is a newly described member of the lipocalin protein family, secreted from specific granules of human neutrophils upon activation of the cells. Its ability to bind the bacterial chemotactic formylpeptide FMLP indicates, that NGAL may have modulatory effects on the immune response. We here describe monoclonal and polyclonal antibodies against NGAL, which can be used for Western blotting and immunohistochemistry, and furthermore describe two ELISAs using either exclusively the polyclonal anti-NGAL antibodies or the polyclonal and monoclonal antibodies in combination. The assays are equally specific, reproducible, accurate, and sensitive, with a detection limit of 32 ng/l. The antibodies and assays will be valuable tools in the future investigation of NGAL expression in inflammatory and malignant disorders and in the elucidation of the function of NGAL as a modulator of the inflammatory response.

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Citations

Mar 30, 2007·Inflammatory Bowel Diseases·Matam Vijay-KumarAndrew T Gewirtz
Oct 1, 2011·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Parnell C MattisonPatricio E Ray
Aug 7, 1997·Journal of Immunological Methods·O SørensenN Borregaard
Jan 5, 2000·Journal of Immunological Methods·O Sørensen, N Borregaard
Sep 11, 2010·Clinical Journal of the American Society of Nephrology : CJASN·Linjun CaiShengyuan Xu
Mar 13, 2009·Clinical Journal of the American Society of Nephrology : CJASN·Angel A Soler-GarcíaPatricio E Ray
Mar 25, 2014·Blood Cancer Journal·F EmmertsenN Borregaard
Sep 23, 2014·The Journal of Clinical Investigation·Ole E SørensenNiels Borregaard
Apr 14, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Linjun CaiPer Venge
May 4, 2006·International Journal of Cancer. Journal International Du Cancer·Stefano CaramutaDomenico Delia
Sep 9, 2005·European Journal of Haematology·Pia KlausenNiels Borregaard
Mar 13, 2015·Proteomics. Clinical Applications·Sofia PerazzoPatricio E Ray
Apr 26, 2008·Journal of Korean Medical Science·Jeung-Hoon LeeJang-Kyu Park
Oct 30, 2009·FEMS Immunology and Medical Microbiology·Erin E JohnsonBobby J Cherayil
Jul 25, 2008·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Gebhard WagenerH Thomas Lee
Jun 10, 2005·Journal of Leukocyte Biology·Mikkel FaurschouNiels Borregaard
Aug 21, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·J Gregory MareshRalph V Shohet
Sep 4, 2009·Virchows Archiv : an International Journal of Pathology·Warner Alpízar-AlpízarBoye Schnack Nielsen
Sep 26, 2008·American Journal of Physiology. Renal Physiology·Eugenia VinuesaGeorgina Hotter
May 12, 2004·Journal of Leukocyte Biology·Carsten Utoft NiemannNiels Borregaard
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matam Vijay-KumarAndrew T Gewirtz
Apr 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jack B CowlandNiels Borregaard
Mar 5, 2019·Cancer Biomarkers : Section a of Disease Markers·Aneta Cymbaluk-PłoskaJanusz Menkiszak
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ole E SørensenNiels Borregaard
Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jack B CowlandNiels Borregaard
Dec 12, 2018·Frontiers in Immunology·Sara Louise DahlJack Bernard Cowland
Jan 7, 2020·Cellular and Molecular Life Sciences : CMLS·Sayan GhoshDebasish Sinha

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