Characterization of two engineered dimeric Zika virus envelope proteins as immunogens for neutralizing antibody selection and vaccine design

The Journal of Biological Chemistry
Chunpeng YangRui Gong


The envelope protein of Zika virus (ZIKV) exists as a dimer on the mature viral surface and is an attractive antiviral target because it mediates viral entry. However, recombinant soluble wild-type ZIKV envelope (wtZE) might preferentially exist as monomer (monZE). Recently, it has been shown that the A264C substitution could promote formation of dimeric ZIKV envelope protein (ZEA264C), requiring further characterization of purified ZEA264C for its potential applications in vaccine development. We also noted that ZEA264C, connected by disulfide bond, might be different from the noncovalent native envelope dimer on the virion surface. Because the antibody Fc fragment exists as dimer and is widely used for fusion protein construction, here we fused wtZE to human immunoglobulin G1 (IgG1) Fc fragment (ZE-Fc) for noncovalent wtZE dimerization. Using a multistep purification procedure, we separated dimeric ZEA264C and ZE-Fc, revealing that they both exhibit typical β-sheet-rich secondary structures and stabilities similar to those of monZE. The binding activities of monZE, ZEA264C, and ZE-Fc to neutralizing antibodies targeting different epitopes indicated that ZEA264C and ZE-Fc could better mimic the native dimeric status, especiall...Continue Reading


Sep 12, 2006·The Journal of General Virology·Karin Stiasny, Franz X Heinz
Jan 18, 2011·Nature Biotechnology·Lilin YeXiaoping Zhu
Nov 4, 2011·Viruses·Jolanda M SmitJan Wilschut
Jan 29, 2013·Current Opinion in Virology·Theodore C Pierson, Margaret Kielian
May 3, 2013·Journal of Virology·Victor A KostyuchenkoShee-Mei Lok
Dec 6, 2014·ELife·Luke H ChaoStephen C Harrison
Mar 11, 2016·Journal of Virology·Helen M Lazear, Michael S Diamond
Apr 2, 2016·Science·Devika SirohiRichard J Kuhn
Apr 20, 2016·Nature·Victor A KostyuchenkoShee-Mei Lok
Apr 27, 2016·Lancet·Patrícia BrasilTom Solomon
May 6, 2016·Journal of Virology·Janja BlazevicKarin Stiasny
Jun 25, 2016·Nature·Giovanna Barba-SpaethFélix A Rey
Jun 30, 2016·Lancet·Leonelo E Bautista, Ajay K Sethi
Aug 1, 2016·Cell·Haiyan ZhaoDaved H Fremont
Oct 21, 2016·Archives of Pathology & Laboratory Medicine·Carey L Medin, Alan L Rothman
Feb 24, 2017·Immunity·Dan H BarouchNelson L Michael
Mar 17, 2017·Nature Communications·S Saif HasanMichael G Rossmann
Mar 23, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jucille do Amaral MenesesLindomar José Pena
Apr 7, 2017·JAMA : the Journal of the American Medical Association·Daniel LuceySabrina Sholts
Aug 22, 2017·Frontiers in Microbiology·Ashok MunjalSunil K Joshi
Sep 17, 2017·Biotechnology Advances·Hsiao-Han LinSuh-Chin Wu
Dec 22, 2017·The Journal of Infectious Diseases·Xuping XiePei-Yong Shi
Dec 22, 2017·The Journal of Infectious Diseases·Devika Sirohi, Richard J Kuhn
Jan 13, 2018·Nature Structural & Molecular Biology·S Saif HasanMichael G Rossmann
Jul 1, 2018·Structure·Madhumati SevvanaMichael G Rossmann

Related Concepts

Zika virus (organism)
Antibodies, Viral
Antigenic Specificity
Mice, Inbred BALB C
Hybrid Proteins, Recombinant
Corona Virus Membrane Protein E1
Viral Vaccines
Protein Engineering
Protein Conformation, beta-Strand

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.


Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.