Characterization of Two Metal Binding Lipoproteins as Vaccine Candidates for Enterococcal Infections

PloS One
Felipe Romero-SaavedraJohannes Huebner

Abstract

Enterococcus faecium and faecalis are Gram-positive opportunistic pathogens that have become leading causes of nosocomial infections over the last decades. Especially multidrug resistant enterococci have become a challenging clinical problem worldwide. Therefore, new treatment options are needed and the identification of alternative targets for vaccine development has emerged as a feasible alternative to fight the infections caused by these pathogens. We extrapolate the transcriptomic data from a mice peritonitis infection model in E. faecalis to identify putative up-regulated surface proteins under infection conditions in E. faecium. After the bionformatic analyses two metal binding lipoproteins were identified to have a high homology (>72%) between the two species, the manganese ABC transporter substrate-binding lipoprotein (PsaAfm,) and the zinc ABC transporter substrate-binding lipoprotein (AdcAfm). These candidate lipoproteins were overexpressed in Escherichia coli and purified. The recombinant proteins were used to produce rabbit polyclonal antibodies that were able to induce specific opsonic antibodies that mediated killing of the homologous strain E. faecium E155 as well as clinical strains E. faecium E1162, Enterococcu...Continue Reading

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Citations

Sep 21, 2018·PLoS Pathogens·Cristina Colomer-WinterJosé A Lemos
Jul 10, 2019·Infection and Immunity·Ermioni KalfopoulouJohannes Huebner
Jul 11, 2019·The Journal of Infectious Diseases·F Romero-SaavedraJ Huebner
Sep 3, 2017·The Journal of Infectious Diseases·Diana LaverdeJohannes Huebner
Oct 13, 2020·Frontiers in Microbiology·Minh-Thu NguyenFriedrich Götz
Nov 6, 2020·Cells·Ermioni Kalfopoulou, Johannes Huebner

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Methods Mentioned

BETA
PCR
affinity purification
Assay
ELISA
dissection

Software Mentioned

GraphPad PRISM
Matrix Science
blast
mascot

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