Characterization of two mRNA species encoding human estradiol 17 beta-dehydrogenase and assignment of the gene to chromosome 17.

Journal of Steroid Biochemistry
F LabrieJ Simard

Abstract

Using two 33-mer synthetic oligonucleotides derived from the amino acid sequence of the catalytic site of estradiol 17 beta-dehydrogenase (E2DH) and polyclonal antibodies raised against the enzyme purified from human placenta, clones were isolated from a lambda gt11 human placental cDNA library. A 327-amino acid sequence was deduced from cDNA sequencing. Two mRNA species have been identified in poly(A)+ RNA from human placenta, a major species migrating at 1.3 kb while a minor one is found at approx. 2.2 kb. Primer extension and S1 nuclease analysis indicate that the major mRNA species starts 9-10 nucleotides while the minor mRNA starts 971 nucleotides upstream from the ATG initiating codon, respectively. Sequence analysis of the longest cDNA clone (2092 bp) shows that it possesses identical coding and non-coding sequences in the regions of overlap with the shorter cDNA clones. The 32P-labeled 5' non-coding fragment hybridizes only to the 2.2 kb band, thus providing evidence for the existence of two distinct mRNA species which differ in their 5' noncoding regions. Using hp E2DH-36 cDNA as a probe for in situ hybridization of translocated chromosomes, the human E2DH gene was localized to the q11-q12 region of chromosome 17.

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Citations

Dec 11, 1999·Fertility and Sterility·K M Zeitoun, S E Bulun
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·A M Mensah-NyaganH Vaudry
Aug 5, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Nana SakuraiMasahisa Nakamura
Jun 17, 1998·Journal of Helminthology·W M HominickM Yoshida
May 6, 1998·The Journal of Steroid Biochemistry and Molecular Biology·D PoirierS Auger
Jan 13, 2006·Molecular and Cellular Endocrinology·Serdar E BulunJ Julie Kim
Jun 10, 2009·Frontiers in Neuroendocrinology·Jean Luc Do RegoHubert Vaudry

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