PMID: 37613Jun 1, 1979Paper

Characterization of two new enzymatic activities of the rat ventral prostate: 5 alpha-androstane-3 beta, 17 beta-diol 6 alpha-hydroxylase and 5 alpha-androstane-3 beta, 17 beta-diol 7 alpha-hydroxylase

Steroids
J T IsaacsD S Coffey

Abstract

This study has characterized two new enzymatic hydroxylase activities specific for 5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol) in the rat ventral prostate: 5 alpha-androstane-3 beta, 17 beta-diol 6 alpha-hydroxylase (6 alpha-hydroxylase) and 5 alpha-androstane-3 beta, 17 beta-diol 7 alpha-hydroxylase (7 alpha-hydroxylase). Both of these irreversible hydroxylase activities require NADPH and are localized in the microsomal fraction of the prostate. The apparent Km for 3 beta-diol is 2.5 microM for both the 6 alpha- and 7 alpha-hydroxylase activities. The apparent Km for NADPH is 7.6 microM for the 6 alpha-hydroxylase and 7.0 microM for the 7 alpha-hydroxylase. The pH optimum for both activities is 7.4. Several steroid inhibitors of these hydroxylase activities in vitro were identified including cholesterol, progesterone, and estradiol. Estradiol was found in vitro to be a noncompetitive inhibitor (Ki = 5 microM). Injection of estradiol into intact male rats, simultaneously receiving exogenous testosterone, also produced a significant lowering of the 6 alpha-plus 7 alpha-hydroxylase activities. Both the 6 alpha- and 7 alpha-hydroxylase were found to be androgen sensitive. Following castration there is a rapid decrease in...Continue Reading

Citations

Jun 28, 2003·FEBS Letters·Zhang WeihuaJan-Ake Gustafsson
May 9, 2009·International Journal of Urology : Official Journal of the Japanese Urological Association·Takayuki KurosakiTadaichi Kitamura
Feb 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yoko OmotoJan-Ake Gustafsson
Apr 12, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Robert Morfin
Feb 1, 1995·Molecular and Cellular Endocrinology·C Guillemette, A Bélanger
Oct 26, 2002·Steroids·Richard Lathe
Mar 1, 1988·Journal of Steroid Biochemistry·J Orlowski, A F Clark
Jun 1, 1982·Molecular and Cellular Endocrinology·M TenniswoodA F Clark
Jul 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·R Morfin, G Courchay
Jul 22, 2010·Trends in Endocrinology and Metabolism : TEM·Nobuhiro SugiyamaJan-Ake Gustafsson

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