Characterization of Two Novel Bacteriophages Infecting Multidrug-Resistant (MDR) Acinetobacter baumannii and Evaluation of Their Therapeutic Efficacy in Vivo

Frontiers in Microbiology
Kyoungeun ChaHeejoon Myung

Abstract

Acinetobacter baumannii is emerging as a challenging nosocomial pathogen due to its rapid evolution of antibiotic resistance. We report characterization of two novel bacteriophages, PBAB08 and PBAB25, infecting clinically isolated, multidrug-resistant (MDR) A. baumannii strains. Both phages belonged to Myoviridae of Caudovirales as their morphology observed under an electron microscope. Their genomes were double stranded linear DNAs of 42,312 base pairs and 40,260 base pairs, respectively. The two phages were distinct from known Acinetobacter phages when whole genome sequences were compared. PBAB08 showed a 99% similarity with 57% sequence coverage to phage AB1 and PBAB25 showed a 97% similarity with 78% sequence coverage to phage IME_AB3. BLASTN significant alignment coverage of all other known phages were <30%. Seventy six and seventy genes encoding putative phage proteins were found in the genomes of PBAB08 and PBAB25, respectively. Their genomic organizations and sequence similarities were consistent with the modular theory of phage evolution. Therapeutic efficacy of a phage cocktail containing the two and other phages were evaluated in a mice model with nasal infection of MDR A. baumannii. Mice treated with the phage cockt...Continue Reading

References

Jan 1, 1980·Annals of the New York Academy of Sciences·D Botstein
Sep 8, 2005·Journal of Clinical Microbiology·Sergio G BartualFrancisco Rodríguez-Valera
Nov 17, 2007·Nature Reviews. Microbiology·Lenie DijkshoornHarald Seifert
Dec 11, 2008·Methods in Molecular Biology·Andrew M KropinskiRoger P Johnson
Dec 11, 2008·Methods in Molecular Biology·Paul Hyman, Stephen T Abedon
May 10, 2012·FEMS Microbiology Reviews·Michael J McConnellJerónimo Pachón
Jan 16, 2013·Trends in Microbiology·Meritxell García-QuintanillaMichael J McConnell
May 18, 2013·Archives of Virology·Min Soo KimHeejoon Myung
Jun 12, 2014·Journal of Applied Microbiology·K ParkH Myung
Nov 21, 2014·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·VinodKumar Chickmangalure ShivaswamyUsha Nandini Pushparaj
Feb 3, 2015·Seminars in Respiratory and Critical Care Medicine·Yohei DoiAnton Y Peleg
Jul 17, 2015·The Journal of Antimicrobial Chemotherapy·Tzu-Wen HuangShih-Feng Tsai
Jul 30, 2015·Journal of Medical Microbiology·Felipe F TuonAlexandre B Merlini
Dec 30, 2015·Journal of Bacteriology·Brent S WeberMario F Feldman
Mar 10, 2016·Antimicrobial Agents and Chemotherapy·Thomas KrahnAndreas Schlüter
Jul 20, 2016·Antimicrobial Agents and Chemotherapy·James M RegeimbalEric R Hall
Mar 30, 2017·Frontiers in Cellular and Infection Microbiology·Chang-Ro LeeSang Hee Lee
Aug 23, 2017·World Journal of Gastrointestinal Pharmacology and Therapeutics·Derek M LinHenry C Lin
Aug 26, 2017·Microbiology Spectrum·Stephen T Abedon

❮ Previous
Next ❯

Citations

May 17, 2019·Virologica Sinica·Natalia BagińskaEwa Jończyk-Matysiak
Sep 20, 2019·Future Microbiology·Andrzej GórskiJan Borysowski
Dec 18, 2019·Current Pharmaceutical Biotechnology·Niran Adhikari, Krishna Prasad Acharya
Dec 12, 2018·Archives of Virology·Hyun Keun OhHeejoon Myung
Nov 9, 2018·Frontiers in Cellular and Infection Microbiology·Lucy L FurfaroBarbara J Chang
Dec 3, 2020·Expert Review of Anti-infective Therapy·Amrita PatilSunil Saroj
Jan 4, 2021·Current Opinion in Biotechnology·Katarzyna Gembara, Krystyna Dąbrowska
Nov 18, 2020·International Journal of Molecular Sciences·Corneliu Ovidiu VrancianuMariana Carmen Chifiriuc
Jan 21, 2021·International Journal of Molecular Sciences·Abid Ali KhanHans-Peter Deigner
Jul 3, 2021·Antibiotics·Antonio Broncano-LavadoMeritxell García-Quintanilla
Jul 2, 2021·Environmental Microbiology Reports·Sandhya Rai, Amod Kumar
Nov 26, 2021·Applied Microbiology and Biotechnology·Kanika BhargavaNeelam Jain

❮ Previous
Next ❯

Datasets Mentioned

BETA
MG366114

Methods Mentioned

BETA
PBAB
electron microscopy

Software Mentioned

BLASTN
Mega
BLAST
MAUVE
ORF Finder

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.